Diversity of Bacillus thuringiensis environmental isolates showing larvicidal activity specific for mosquitoes
Ishii, Takaaki and Ohba, Michio,, 139, 2849-2854 (1993),
doi = https://doi.org/10.1099/00221287-139-11-2849,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Seven mosquito-specific strains of Bacillus thuringiensis isolated in Japan were examined for their flagellar(H)antigenicities and the properties of parasporal inclusion proteins. They were assigned to six H serovars: serovar sis (H 3ade); serovar canadensis (H 5ac); serovar darmstadiensis (H 10); serovar kyushuensis (H): serovar shandongiensis (H 22); and an undescribed serovar belonging to H serotype 20. Purified parasporal inclusions exhibited moderate mosquito larvicidal activities with LC50 values ranging from 1 μg ml−1 to 10 μg ml−1. The inclusions of these strains consisted of highly heterogeneous multiple protein components, and five distinct patterns were evident in their SDS-PAGE profiles. Antibodies against kyushuensis inclusion proteins were reactive with all strains to varying degrees, while israelensis antibodies gave only relatively weak reactions with the seven strains. Similarity in antibody-binding profiles was associated with similarity in SDS-PAGE profiles. In a given strain, different antisera gave altered immunoblot profiles. Haemolytic activity was shown by solubilized parsporal inclusion proteins of five of the seven strains.,
language=,
type=