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Volume 139, Issue 6

Induction of extracellular proteinase in Free

Abstract

Pulse-chase experiments indicated that the extracellular proteinase (EPR) of originates as a 45 kDa precursor protein which is processed to a 43 kDa protein prior to secretion. Secretion was routinely stimulated in EPR induction medium which contains bovine serum albumin (BSA) and glucose. Although EPR was not induced without glucose as a carbon source, EPR secretion was induced without the addition of BSA or other nitrogen sources. Furthermore, it was shown that EPR production was not induced at pH > 6·0, irrespective of the presence of a nitrogen source. This suggests that medium pH may act directly upon EPR induction, and not as a secondary effect of the nitrogen supply from EPR-mediated protein digestion, which exhibited a pH optimum of around pH 3·5. When germ tube induced cells were transferred to EPR induction medium, EPR was not induced. Thus, EPR production and germ tube formation may not be induced by the same conditions. We speculate that EPR production and germ tube formation do not co-operate in the invasive process but play different and separate roles.

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  • Published Online:
© Society for General Microbiology 1993
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1993-06-01
2024-04-19
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References

  1. Banerjee A., Ganesan K., Datta A. 1991; Induction of secretory acid proteinase in Candidaalbicans . Journal of General Microbiology 137:2455–2461
     [Google Scholar]
  2. Bogonez E., Satrustegui J., Machado A. 1985; Regulation by ammonium of glutamate dehydrogenase (NADP+) from Saccharomyces cerevisiae . Journal of General Microbiology 131:1425–1432
     [Google Scholar]
  3. Borg M., Ruchel R. 1988; Expression of extracellular acid proteinase by proteolytic Candida spp. during experimental infection of oral mucosa. Infection and Immunity 56:626–631
     [Google Scholar]
  4. Crandall M., Edwards J. E. 1987; Segregation of proteinasenegative mutants from heterozygous Candida albicans . Journal of General Microbiology 133:2817–2824
     [Google Scholar]
  5. Cutler J. E. 1991; Putative virulence factors of Candida albicans . Annual Review of Microbiology 45:187–218
     [Google Scholar]
  6. Germaine G. R., Tellefson L. M. 1981; Effect of pH and human saliva on protease production by Candida albicans . Infection and Immunity 31:323–326
     [Google Scholar]
  7. Hattori M., Yoshiura K., Negi M., Ogawa H. 1984; Keratinolytic proteinase produced by Candida albicans . Journal of Medical and Veterinary Mycology 22:175–183
     [Google Scholar]
  8. Holmes A. R., Collings A., Farnden K. J. F., Shepherd M. G. 1989; Ammonium assimilation by Candida albicans and other yeasts: evidence for activity of glutamate synthase. Journal of General Microbiology 135:1423–1430
     [Google Scholar]
  9. Homma M., Kutsukake K., Iino T. 1985; Structural genes for flagellar hook-associated proteins in Salmonella typhimurium . Journal of Bacteriology 163:464–471
     [Google Scholar]
  10. Homma M., Kanbe T., Chibana H., Tanaka K. 1992; Detection of intracellular forms of secreted aspartic proteinase in Candida albicans . Journal of General Microbiology 138:627–633
     [Google Scholar]
  11. Hube B., Turver C. J., Odds F. C., Eiffert H., Boulnois G. J., Kochel H., Ruchel R. 1991; Sequence of the Candida albicans gene encoding the secretory aspartate proteinase. Journal of Medical and Veterinary Mycology 29:129–132
     [Google Scholar]
  12. Ishiguro A., Homma M., Sukai T., Higashide K., Torii S., Tanaka K. 1992a; Detection of antigen components of Candida albicans by reaction with antibody from candidial vaginitis patients and healthy females. Journal of Medical and Veterinary Mycology 30:281–292
     [Google Scholar]
  13. Ishiguro A., Homma M., Torii S., Tanaka K. 1992b; Identification of Candida albicans antigens reacted with IgE antibody of human sera. Infection and Immunity 60:1550–1557
     [Google Scholar]
  14. Iwaguchi S.-I., Homma M., Tanaka K. 1990; Variation in the electrophoretic karyotype analysed by the assignment of DNA probes in Candida albicans . Journal of General Microbiology 136:2433–2442
     [Google Scholar]
  15. Kobayashi I., Kondoh Y., Shimizu K., Tanaka K. 1989; A role of secreted proteinase of Candida albicans for the invasion of chick chorio-allantoic membrane. Microbiology and Immunology 33:709–719
     [Google Scholar]
  16. Macdonald F., Odds F. C. 1980; Purified Candida albicans proteinase in the serological diagnosis of systemic candidosis. Journal of the American Medical Association 243:2409–2411
     [Google Scholar]
  17. Marzluf G. A. 1981; Regulation of nitrogen metabolism and gene expression in fungi. Microbiological Reviews 45:437–461
     [Google Scholar]
  18. Matsuda K. 1986; Influence of nitrogen source, pH of media and Candida albicans- producing proteinase (CAPP) ( = keratinolytic proteinase; KPase)on the growth of C. albicans . Japanese Journal of Medical Mycology 27:100–106
     [Google Scholar]
  19. Mazon M. J. 1978; Effect of glucose starvation on the nicotinamide adeninedinucleotide phosphate-dependent glutamate dehydrogenase of yeast. Journal of Bacteriology 133:780–785
     [Google Scholar]
  20. Mazon M. J., Hemmings B. A. 1979; Regulation of Saccharomyces cerevisiae nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase by proteolysis during carbon starvation. Journal of Bacteriology 139:686–689
     [Google Scholar]
  21. Negi M., Tsuboi R., Matsui T., Ogawa H. 1984; Isolation and characterization of proteinase from Candida albicans: substrate specificity. Journal of Investigative Dermatology 83:32–36
     [Google Scholar]
  22. Odds F. C. 1985; Morphogenesis in Candida albicans . CRC Critical Reviews in Microbiology 12:45–93
     [Google Scholar]
  23. Odds F. C. 1988a; Morphogenesis in Candida, with special reference to C. albicans . In Candida and Candidosis pp. 42–59 London: Bailliere Tindall;
     [Google Scholar]
  24. Odds F. C. 1988b; Ecology of Candida and epidemiology of candidosis. In Candida and Candidosis pp. 68–92 London: Bailliere Tindall;
     [Google Scholar]
  25. Odds F. C. 1988c; Pathogenesis of Candidosis. In Candida and Candidosis pp. 252–278 London: Bailliere Tindall;
     [Google Scholar]
  26. Ray T. L., Payne C. D. 1988; Scanning electron microscopy of epidermal adherence and cavitation in murine candidiasis: a role for Candida acid proteinase. Infection and Immunity 56:1942–1949
     [Google Scholar]
  27. Reid G. A. 1991; Protein targeting in yeast. Journal of General Microbiology 137:1765–1773
     [Google Scholar]
  28. Remold H., Fasolk H., Staib F. 1968; Purification and characterization of a proteolytic enzyme from Candida albicans . Biochimica et Biophysica Acta 167:399–406
     [Google Scholar]
  29. Ross I. K., De Bernardis F., Emerson G. W., Cannone A., Sullivan P. A. 1990; The secreted aspartate proteinase of Candida albicans: physiology of secretion and virulence of a proteinase-deficient mutant. Journal of General Microbiology 136:687–694
     [Google Scholar]
  30. Samaranayake L. P., Hughes A., Macfarlane T. W. 1984; The proteolytic potential of Candida albicans in human saliva supplemented with glucose. Journal of Medical Microbiology 17:13–22
     [Google Scholar]
  31. Shimizu K., Kondoh Y., Tanaka K. 1987; Proteinase production and pathogenicity of Candida albicans . Microbiology and Immunology 31:1045–1060
     [Google Scholar]
  32. Uno I., Matsumoto K., Adachi K., Ishikawa T. 1984; Regulation of NAD-dependent glutamate dehydrogenase by protein kinases in Saccharomyces cerevisiae . Journal of Biological Chemistry 259:1288–1293
     [Google Scholar]
  33. Wiame J. M., Grenson M., Arst H. N. 1985; Nitrogen catabolite repression in yeasts and filamentous fungi. Advances in Microbial Physiology 26:1–88
     [Google Scholar]
  34. Wingard J., Dick J., Merz W., Sandford G., Saral R., Burns W. 1982; Differences in virulence of clinical isolates of Candida tropicalis and Candida albicans in mice. Infection and Immunity 37:833–836
     [Google Scholar]
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Induction of extracellular proteinase in Candida albicans
Microbiology 139, 1187 (1993); https://doi.org/10.1099/00221287-139-6-1187
/content/journal/micro/10.1099/00221287-139-6-1187
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