f Transcriptional regulation of an endoglucanase and a cellodextrinase gene in Ruminococcus flavefaciens FD-1
- Authors: Wenyen Wang, Sharon J. Reid, Jennifer A. Thomson*
- *Author for correspondence. Tel. (21) 650 3270; fax (21) 650 4023; e-mail firstname.lastname@example.org.
- First Published Online: 01 June 1993, Microbiology 139: 1219-1226, doi: 10.1099/00221287-139-6-1219
- Subject: Genetics And Molecular Biology
- Issue Published:
Summary: A gene which encodes a 35 kDa protein with both carboxymethylcellulase and xylanase activity was cloned from Ruminococcus flavefaciens FD-1 and the nucleotide sequence determined. The FD-1 gene, celE, and the celA gene, which encodes a cellodextrinase, were used as probes to analyse transcription in R. flavefaciens grown under different conditions. Transcription of both genes was induced when cellulose was added to cells growing in cellobiose. This induction continued after cellulose depletion and after cell division had ceased. Transcription of both genes was also induced by cellotriose, although the effect was not as pronounced as induction by cellulose and was greater for the celA gene than for the celE gene.
© Society for General Microbiology 1993 | Published by the Microbiology Society
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/content/journal/micro/10.1099/00221287-139-6-1219dcterms_title,dcterms_subject-pub_serialIdent:journal/micro AND -contentType:BlogPost104
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