f phytogeny and phenotypic characterization of the stalk-forming and iron-oxidizing bacterium Gallionella ferruginea
- Authors: Lqtta Hallbeck*, Fredrik Ståhl, Karsten Pedersen
- * Author for correspondence. Tel. 46 31 418700; fax 46 31 826790.
- Microbiology, July 1993 139: 1531-1535, doi: 10.1099/00221287-139-7-1531
- Subject: Systematics
- Published Online:
Summary: The 16S rRNA gene of Gallionella feiruginea was amplified by polymerase chain reaction and sequenced by direct double-stranded sequencing. The phylogenetic analysis placed G. feiruginea in the β-group of the Proteobacteria, with 90.0% similarity to Nitrosolobus multiformis and 88.6% to Rhodocyclus purpureas. The published phenotypic characteristics of G. ferruginea were compiled and supplemented with growth experiments using ferrous iron, thiosulphate and sulphide as electron donor, and nitrate as nitrogen source. G. ferruginea is a Gram-negative, curved bacterium with one polar fiagellum. It grows auto- and mixotrophically with CO2, glucose, fructose and sucrose as carbon sources, ferrous iron as an electron donor and ammonium or nitrate as nitrogen sources. Two G. feiruginea specific oligonucleotide probes are suggested. An iron-oxidizing bacterium without stalk-forming ability, but with the same growth pattern as G. ferruginea, was identified as G. ferruginea by comparison of highly variable parts of the 16S rRNA gene. This indicates that the stalk is not essential for growth.
© Society for General Microbiology 1993 | Published by the Microbiology Society
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