f Synthesis in vitro of crystalline chitin by a solubilized enzyme from the cellulosic fungus Saprolegnia monoica
- Authors: Lucien Gay*, Henri Chanzy, Vincent Bulone, Vincent Girard, Michel Fèvre
- *Author for correspondence. Tel. 72 44 85 44; fax 72 43 11 81.
- First Published Online: 01 September 1993, Microbiology 139: 2117-2122, doi: 10.1099/00221287-139-9-2117
- Subject: Biochemistry
- Issue Published:
SUMMARY: Enriched preparations of chitin synthase were obtained from cell homogenates from Saprolegnia monoica. Chitin synthase was solubilized from a mixed membrane fraction by two successive digitonin treatments. Glycerol gradient centrifugation of the solubilized proteins separated the chitin synthase activity from the majority of proteins and from β-1,3 and β-1,4 glucan synthases. The properties of chitin synthase from this Oomycete fungus are similar to those reported for the enzymes of chitinous fungi. The solubilized enzyme catalysed the synthesis in vitro of spindle-like crystals of chitin. Biophysical analysis (by electron and X-ray diffractometry and infrared spectroscopy) demonstrated that the polymer synthesized in vitro was α-chitin. These results and those previously reported demonstrate unambiguously that chitin synthase and chitin are normal components of the cell wall of the cellulosic fungus S. monoica.
© Society for General Microbiology 1993 | Published by the Microbiology Society
Article metrics loading...
Full text loading...
Author and Article Information
/content/journal/micro/10.1099/00221287-139-9-2117dcterms_title,dcterms_subject,pub_serialTitlepub_serialIdent:journal/micro AND -contentType:BlogPost104
/content/journal/micro/10.1099/00221287-139-9-2117dcterms_title,dcterms_subject-pub_serialIdent:journal/micro AND -contentType:BlogPost104
Figure data loading....