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Volume 140, Issue 4

Effects of oxygen limitation on sugar metabolism in yeasts: a continuous-culture study of the Kluyver effect Free

Abstract

Growth and metabolite formation were studied in oxygen-limited chemostat cultures of CBS 8066 and CBS 621 growing on glucose or maltose at a dilution rate of 0·1 h With either glucose or maltose could be grown under dual limitation of oxygen and sugar. Respiration and alcoholic fermentation occurred simultaneously and the catabolite fluxes through these processes were dependent on the magnitude of the oxygen feed. could also be grown under dual limitation of glucose and oxygen. However, at very low oxygen feed rates (i.e. below 4 mmol l h) growth was limited by oxygen only, as indicated by the high residual glucose concentration in the culture. In contrast to could not be grown anaerobically at a dilution rate of 0·1 h. With absence of oxygen resulted in wash-out, despite the presence of ergosterol and Tween-80 in the growth medium. The behaviour of with respect to maltose utilization in oxygen-limited cultures was remarkable: alcoholic fermentation did not occur and the amount of maltose metabolized was dependent on the oxygen supply. Oxygen-limited cultures of growing on maltose always contained high residual sugar concentrations. These observations throw new light on the so-called Kluyver effect. Apparently, maltose is a non-fermentable sugar for CBS 621, despite the fact that it can serve as a substrate for growth of this facultatively fermentative yeast. This is not due to the absence of key enzymes of alcoholic fermentation. Pyruvate decarboxylase and alcohol dehydrogenase were present at high levels in maltose-utilizing cells of grown under oxygen limitation. It is concluded that the Kluyver effect, in growing on maltose, results from a regulatory mechanism that prevents the sugar from being fermented. Oxygen is not a key factor in this phenomenon since under oxygen limitation alcoholic fermentation of maltose was not triggered.

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Keyword(s): Candida utilis , Kluyver effect , oxygen limitation , Saccharomyces cerevisiae and sugar metabolism
© Society for General Microbiology 1994
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1994-04-01
2024-04-23
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Effects of oxygen limitation on sugar metabolism in yeasts: a continuous-culture study of the Kluyver effect
Microbiology 140, 703 (1994); https://doi.org/10.1099/00221287-140-4-703
/content/journal/micro/10.1099/00221287-140-4-703
/content/journal/micro/10.1099/00221287-140-4-703
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