%0 Journal Article %A Pronk, Jack T. %A van der Linden-Beuman, Annelies %A Verduyn, Cornelis %A Scheffers, W. Alexander %A van Dijken, Johannes P. %T Propionate metabolism in Saccharomyces cerevisiae: implications for the metabolon hypothesis %D 1994 %J Microbiology, %V 140 %N 4 %P 717-722 %@ 1465-2080 %R https://doi.org/10.1099/00221287-140-4-717 %K metabolic channelling %K Saccharomyces cerevisiae %K 2-methylcitrate pathway %K propionate metabolism %I Microbiology Society, %X Aerobic, glucose-limited chemostat of Saccharomyces cerevisiae CBS 8066 cometabolized propionate when this compound was added to the reservoir medium. Co-metabolism of propionate led to an increase of the biomass and protein yields. Attempts to grow S. cerevisiae on propionate as a sole source of carbon and energy were not successful. Activities of propionyl-CoA synthetase in cell-free extracts were sufficient to account for the rates of propionate consumption observed in the chemostat cultures. Activities of propionyl-CoA carboxylase, a key enzyme of the methylmalonyl-CoA pathway of propionate metabolism, were negligible. In contrast, activities of 2-methylcitrate synthase, a key enzyme activity of the 2-methylcitrate pathway of propionate metabolism, increased substantially with increasing propionateto-glucose ratios in the reservoir media, and were sufficient to account for the propionate consumption rates observed in the chemostat cultures. This suggested that the 2-methylcitrate pathway is the major pathway of propionate metabolism in S. cerevisiae. In the literature, labelling patterns observed after incubation of this yeast with [3-13C]propionate have been interpreted as evidence for channelling of tricarboxylic acid (TCA) cycle intermediates, possibly as a consequence of the organization of TCA cycle enzymes in a metabolon. However, this interpretation of 13C-labelling patterns rested on the assumption that propionate metabolism in S. cerevisiae occurs via the methylmalonyl-CoA pathway. Since the distribution of 13C in alanine reported in the literature is fully compatible with a major role of the 2-methylcitrate pathway in propionate metabolism, it cannot be interpreted as evidence for the existence of a TCA cycle metabolon in S. cerevisiae. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-140-4-717