1887

Abstract

Carnocin UI49, a lantibiotic produced by shows bactericidal activity against many lactic acid bacteria. This paper describes the results of a study on the mode of action of carnocin UI49. It has previously been observed that nisin-producing subsp. are at least 10-fold more sensitive to carnocin UI49 relative to other lactic acid bacteria. Addition of carnocin UI49 to cells of subsp. NZ9700 resulted in a dissipation of the membrane potential and a rapid hydrolysis of internal ATP. These results suggest that carnocin UI49 may act at the cytoplasmic membrane. The correlation between production of and/or immunity to nisin and sensitivity to carnocin of subsp. strains was further investigated. Several transformed subsp. strains carrying a fragment of the nisin gene cluster were tested for their sensitivity to carnocin UI49 and their immunity to nisin. The results suggest that NisP, which is one of the membrane-associated proteins involved in the production of nisin acts as receptor for carnocin UI49. This may facilitate the binding and/or insertion of carnocin UI49 into the cytoplasmic membrane thus increasing its bactericidal activity. Lantibiotic-producing and non-producing mutants of and did not show a difference in sensitivity to carnocin UI49. The proposed receptor-mediated action of carnocin UI49 at the cytoplasmic membrane therefore seems to be specific for the nisin-producing strains.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-140-6-1443
1994-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/6/mic-140-6-1443.html?itemId=/content/journal/micro/10.1099/00221287-140-6-1443&mimeType=html&fmt=ahah

References

  1. Abee T., Gao F. H., Konings W. N. 1991; The mechanism of action of the lantibiotic nisin in artificial membranes. In Nisin and Novel Lantibiotics pp. 373–385 Edited by Jung G., Sahl H.-G. Leiden: Escom;
    [Google Scholar]
  2. Andersson R. 1986; Inhibition of Staphylococcus aureus and spheroplasts of Lactobacillus plantarum. Int J Food Microbiol 3:149–160
    [Google Scholar]
  3. Anonymous. 1989 Countries where specific approval exists for the use of nisin Technical Information Leaflet no. 4/89/11. Aplin and Barrett Ltd
    [Google Scholar]
  4. Benz R., Jung G., Sahl H.-G. 1991; Mechanism of channel formation by lantibiotics in black lipid membranes. In Nisin and Novel Lantibiotics pp. 422–433 Edited by Jung G., Sahl H.-G. Leiden: Escom;
    [Google Scholar]
  5. Blight M.A., Holland I. B. 1990; Structure and function of haemolysin B, P-glycoprotein and other members of a novel family of translocators. Mol Microbiol 4:873–880
    [Google Scholar]
  6. De Vos W. M., Mulders J. W. M., Siezen R. J., Hugenholtz J, Kuipers O. P. 1993; Properties of nisin Z and distribution of its gene, nis Z, in Lactococcus lactis. Appl Environ Microbiol 59:213–218
    [Google Scholar]
  7. Delves-Broughton J. 1990; Nisin and its uses as a food preservative. Food Technol 44:100–117
    [Google Scholar]
  8. Driessen A.J.M., Kodde J., de Jong S., Konings W. N. 1987; Neutral amino acid transport by membrane vesicles of Streptococcus cremoris is subjected to regulation of the internal pH. J Bacteriol 169:2748–2754
    [Google Scholar]
  9. Engelke G., Gutowski-Eckel Z., Hammelmann M., Entian K.-D. 1992; Biosynthesis of the lantibiotic nisin: genomic organization and membrane localization of the Nis B protein. Appl Environ Microbiol 58:3730–3743
    [Google Scholar]
  10. Gao F. H., Abee T., Konings W. N. 1991; Mechanism of action of the peptide antibiotic nisin in liposomes and cytochrome c oxidase-containing proteoliposomes. Appl Environ Microbiol 57:2164–2170
    [Google Scholar]
  11. Garcera M.J.G., Elferink M. G. L., Driessen A. J. M., Konings W. N. 1993; In vitro pore-forming activity of the lantibiotic nisin. Role of protonmotive force and lipid composition. Eur J Biochem 216:417–422
    [Google Scholar]
  12. Gasson M.J. 1983; Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J Bacteriol 154:1–9
    [Google Scholar]
  13. Guihard G., Benedetti H., Besnard M., Letellier L. 1993; Phosphate efflux through the channels formed by colicins and phage T5 in Escherichia coli cells is responsible for the fall in cytoplasmic ATP. J Biol Chem 268:17775–17780
    [Google Scholar]
  14. Hoover D., Steenson L. 1993 Bacteriocins of Lactic Acid Bacteria New York: Academic Press;
    [Google Scholar]
  15. Jung G. 1991; Lantibiotics: a survey. In Nisin and Novel Lantibiotics pp. 1–34 Edited by Jung G., Sahl H.-G. Leiden: Escom;
    [Google Scholar]
  16. Klaenhammer T.R. 1993; Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev 12:39–86
    [Google Scholar]
  17. Kuipers O. P., Beerthuyzen M. M., Siezen R. J., De Vos W. M. 1993; Characterization of the nisin gene cluster wAABTCIPR of Lactococcus lactis-, requirement of expression of the nis A and nisi genes for development of immunity. Eur J Biochem 216:281–291
    [Google Scholar]
  18. Liu W., Hansen J. N. 1990; Some chemical and physical properties of nisin. Appl Environ Microbiol 56:2551–2558
    [Google Scholar]
  19. Mortvedt C., Nissen-Meyer J., Sletten K., Nes I. F. 1991; Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45. Appl Environ Microbiol 57:1829–1834
    [Google Scholar]
  20. Ojcius D.M., Young D.-E. . 1991; Cytolytic pore-forming proteins and peptides: is there a common structural motive?. Trends Biochem Sci 16:225–229
    [Google Scholar]
  21. Piard J.C., Muriana P. M., Desmazeaud M. J., Klaenhammer T. R. 1992; Purification and partial characterization of lacticin 481, a lantionine-containing bacteriocin produced by Lactococcus lactis subsp lactis CNRZ 481. Appl Environ Microbiol 58:279–284
    [Google Scholar]
  22. Poolman B. 1993; Energy transduction in lactic acid bacteria. FEMS Microbiol Rev 12:125–148
    [Google Scholar]
  23. Rauch P.J.G., De Vos W. M. 1992; Characterization of the novel nisin-sucrose conjugative transposon Tn5276 and its insertion in Lactococcus lactis. J Bacteriol 174:1280–1287
    [Google Scholar]
  24. Rauch P.J.G., Beerthuyzen M. M., De Vos W. M. 1991; Molecular analysis and evolution of conjugative transposons encoding nisin production and sucrose fermentation in Lactococcus lactis. . In Nisin and Novel Lantibiotics pp. 243–249 Edited by Jung G., Sahl H.-G. Leiden: Escom;
    [Google Scholar]
  25. Ray B. 1992; Bacteriocins of starter culture bacteria as food preservatives: an overview. In Food Preservatives of Microbial Origin pp. 178–205 Edited by Ray B., Daeschel M. . Florida: CRC Press;
    [Google Scholar]
  26. Reis M., Sahl H.-G. 1991; Genetic analysis of the producer self-protection mechanism (“immunity”) against Pep-5. In Nisin and Novel Lantibiotics pp. 320–331 Edited by Jung G., Sahl H.-G. Leiden: Escom;
    [Google Scholar]
  27. Ruhr E., Sahl H.-G. 1985; Mode of action of the peptide antibiotic nisin and the influence on the membrane potential of whole cells and on cytoplasmic and artificial membrane vesicles. Antimicrob Agents Chemother 27:841–845
    [Google Scholar]
  28. Sahl H.G., Kordel M., Benz R. 1987; Voltage dependent depolarization of bacterial membranes and artificial lipid bilayers by the peptide antibiotic nisin. Arch Microbiol 149:120–124
    [Google Scholar]
  29. Shinbo T., Kama N., Karihara K., Kobataka Y. 1978; PVC- based electrode sensitive to TPP+ as a device to monitor the membrane potential in biological systems. Arch Biochem Biophys 187:414–422
    [Google Scholar]
  30. Stoffels G., Nissen-Meyer J., Gudmundsdottir A., Sletten K., Holo H., Nes I. F. 1992a; Purification and characterization of a new bacteriocin isolated from a Carnobacterium sp. Appl Environ Microbiol 58:1417–1422
    [Google Scholar]
  31. Stoffels G., Nes I. F., Gudmundsdottir A. 1992b; Isolation and properties of a bacteriocin-producing Carnobacterium piscicola isolated from fish. J Appl Bacteriol 73:309–316
    [Google Scholar]
  32. Stoffels G., Sahl H.-G., Gudmundsdottir A. 1993; Carnocin UI49, a potential biopreservative produced by Carnobacterium piscicola. Large scale purification and activity against various Gram- positive bacteria including Listeria sp. Int J Food Microbiol 20:199–210
    [Google Scholar]
  33. Thompson J., Sackett D. L., Donkersloot J. A. 1991; Purification and properties of fructokinase I from Lactococcus lactis. Localization of rrr K on the sucrose-nisin transposon Tn5306. J Biol Chem 266:22626–22633
    [Google Scholar]
  34. Van der Meer J. R., Polman J., Beerthuyzen M. M., Siezen R. J., Kuipers O. P., De Vos W. M. 1993; Characterization of the Lactococcus lactis nisin A operon genes nis V, encoding a subtilin-like serine protease involved in precursor processing, and nisAR, encoding a regulatory protein involved in nisin biosynthesis. J Bacterial 175:2578–2588
    [Google Scholar]
  35. Venema K., Abee T., Haandriksman A. J., Leenhouts K. J., Kok J., Konings W. N., Venema G. 1993; Mode of action of lactococcin B, a thiol-activated bacteriocin from Lactococcus lactis. Appl Environ Microbiol 59:1041–1048
    [Google Scholar]
  36. Vos P., Simons G., Siezen R. J., De Vos W. M. 1989; Primary structure and organization of the gene for a procaryotic, cell envelope-located serine protease. J Biol Chem 264:13579–13585
    [Google Scholar]
  37. Zaman Z., Verwilghen R. L. 1979; Quantitation of proteins solubilized in sodium dodecyl sulfate-mercaptoethanol-Tris electrophoresis buffer. Anal Biochem 100:64–69
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-140-6-1443
Loading
/content/journal/micro/10.1099/00221287-140-6-1443
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error