1887

Abstract

SUMMARY:

The cell wall of Lactobacillus helveticus ATCC 12046 was investigated: intact cells were compared to cells stripped of their surface layer (S-layer) by treatment with LiCl. The protein profile and the amino acid composition of isolated cell walls were compared with those of LiCl-treated walls. The S-layer-forming-protein was tested for the presence of glycosidic residues. Finally, the overall elemental composition of the surface of intact cells, LiCl-treated cells and isolated S-layer-forming-protein was determined by X-ray photoelectron spectroscopy. The data collected by the ensemble of methods employed indicate that the surface of intact cells consists mainly of proteins, with some polysaccharides and teichoic or lipoteichoic acids. The exposed surface of LiCl-treated bacteria is rich in peptidoglycan, teichoic acids (or lipoteichoic acids) and polysaccharide. The extracted material is practically pure protein with traces of some glycosidic residues.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-141-1-11
1995-01-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/1/mic-141-1-11.html?itemId=/content/journal/micro/10.1099/00221287-141-1-11&mimeType=html&fmt=ahah

References

  1. Baddiley J., Davison A. L. 1961; The occurence and location of teichoic acids in lactobacilli. J Gen Microbiol 24:295–299
    [Google Scholar]
  2. Bie R., Sjostrom G. 1975; Autolytic properties of some lactic acid bacteria used in cheese production. Milchwissenschaft 30:739–747
    [Google Scholar]
  3. Botazzi V. 1993 Aspects of lactic acid bacteria biology. I. Growth and lysis of Lactobacillus cells in cheese. In biotechnology and Molecular biology of Lactic Acid bacteria for Improvement of Foods and Feeds Quality pp 81–97 Edited by Zamorani A., Manachini P. L., Botazzi V., Coppola S. Roma: Instituto poligraphito e zeca Dello Stato;
    [Google Scholar]
  4. Büchs J., Mozes N., Wandrey C., Rouxhet P. G. 1988; Cell adsorption control by culture conditions: influence of phosphate on surface properties, flocculation and adsorption behaviour of Corynebacterium glutamkum . Appl Microbiol Biotechnol 29:119–128
    [Google Scholar]
  5. Busscher H. J., Bellon-Fontaine M. -N., Mozes N., van der Mei H. C., Sjollema J., Léonard A. J., Rouxhet P. G., Cerf O. 1990; An interlaboratory comparison of physico-chemical methods for studying the surface properties of microorganisms: application to Streptococcus thermophilus and Leuconostoc mesenteroides . J Microbiol Methods 12:101–115
    [Google Scholar]
  6. Busscher H. J., Handley P. S., Rouxhet P. G., Hesketh L. M., van der Mei H. C. 1991; The relationship between structural and physico-chemical surface properties of tufted Streptococcus sanguis strains. Microbial Cell Surface Analysis: Structural and Physico-chemical Methods pp 317–338 Edited by Mozes N., Handley P. S., Busscher H. J., Rouxhet P. G. NY: VCH;
    [Google Scholar]
  7. Cowan M. M., van der Mei H. C., Rouxhet P. G., Busscher H. J. 1992a; Physico-chemical and structural properties of the surfaces of Peptostreptococcus micros and Streptococcus mitis as compared to those of mutant streptococci, Streptococcus sanguis and Streptococcus salivarius . J Gen Microbiol 138:2707–2714
    [Google Scholar]
  8. Cowan M. M., van der Mei H. C., Rouxhet P. G., Busscher H. J. 1992b; Physicochemical and structural investigation of the surface of some anaerobic subgingival bacteria. Appl Environ Microbiol 58:1326–1334
    [Google Scholar]
  9. Coyette J., Ghuysen J. M. 1970; Structure of the walls of Lactobacillus acidophilus strain 63 AM Gasser. Biochemistry 9:2935–2943
    [Google Scholar]
  10. Cuperus P. L., van der Mei H. C., Reid G., Bruce A. W., Khoury A. E., Rouxhet P. G., Busscher H. J. 1992; The effect of serial passaging of lactobacilli in liquid medium on their physico-chemical and structural surface characteristics. Cells Mater 2:271–280
    [Google Scholar]
  11. Cuperus P. L., van der Mei H. C., Reid G., Bruce A. W., Khoury A. H., Rouxhet P. G., Busscher H. J. 1993; Physicochemical surface characteristics of urogenital and poultry lactobacilli. J Colloid Interface Sci 156:319–324
    [Google Scholar]
  12. De Man J. C., Rogosa M., Sharpe E. 1960; A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135
    [Google Scholar]
  13. Dengis P. B., Gerin P. A., Rouxhet P. G. 1995; XPS analysis of biosurfaces: examination of performances with yeast cells and related model compounds. Colloids Surf B: Biointerfaces (in press)
    [Google Scholar]
  14. Eckman 1993; Quantification of subnanomolar amounts of phosphate bound to seryl and threonyl residues in phosphoproteins using alkaline hydrolysis and malachite green. Anal Biochem 214:138–141
    [Google Scholar]
  15. Gerin P. A., Dufrêne Y., Bellon-Fontain M. -N., Asther M., Rouxhet P. G. 1993; Surface properties of the conidiospores of Phanerochaete chrysosporium and their relevance to pellet formation. J Bacteriol 175:5135–5144
    [Google Scholar]
  16. Gerin P. A., Asther M., Sleytr U. B., Rouxhet P. G. 1994; Detection of rodlets in the outer wall region of conidiospores of Phanerochaete chrysosporium . Can J Microbiol 40:412–416
    [Google Scholar]
  17. Herben P. F. G., Mozes N., Rouxhet P. G. 1990; Variation of the surface properties of Bacillus licheniformis according to age, temperature and aeration. Biochim Biophys Acta 1033:184–188
    [Google Scholar]
  18. Knox K. W., Wicken A. J. 1971; Serological properties of the wall and membrane teichoic acids from Lactobacillus helveticus NCIB 8025. J Gen Microbiol 63:237–248
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  20. Lortal S. 1993; Crystalline surface-layers of the genus Lactobacillus . In Advances in Paracrystalline Surface-layers pp 57–65, Edited by. Beveridge T., Koval S. NY: Plenum Press;
    [Google Scholar]
  21. Lortal S., Rousseau M., Boyaval P., van Heijenoort J. 1991; Cell wall and autolytic system of Lactobacillus helveticus ATCC 12046. J Gen Microbiol 137:549–559
    [Google Scholar]
  22. Lortal S., van Heijenoort J., Gruber K., Sleytr U. B. 1992; S-layer of Lactobacillus helveticus ATCC 12046: isolation, chemical characterization and re-formation after extraction with lithium chloride. J Gen Microbiol 138:611–618
    [Google Scholar]
  23. van der Mei H. C., Léonard A. J., Weerkamp A. H., Rouxhet P. G., Busscher H. J. 1988a; Surface properties of Streptococcus salivarius HB and nonfibrillar mutants: measurements of zeta potential and elemental composition with X-ray photoelectron spectroscopy. J Bacteriol 170:2462–2466
    [Google Scholar]
  24. van der Mei H. C., Léonard A. J., Weerkamp A. H., Rouxhet P. G., Busscher H. J. 1988b; Properties of oral Streptococci relevant for adherence: zeta potential, surface free energy and elemental composition. Colloids Surf 32:297–305
    [Google Scholar]
  25. van der Mei H. C., Genet M. J., Weerkamp A. H., Rouxhet P. G., Busscher H. J. 1989a; A comparison between the elemental surface composition and electrokinetic properties of oral streptococci with and without adsorbed salivary constituents. Arch Oral Biol 34:889–894
    [Google Scholar]
  26. van der Mei H. C., Brokke P., Dankert J., Feijen J., Rouxhet P. G., Busscher H. J. 1989b; Physicochemical surface properties of nonencapsulated and encapsulated coagulase-negative staphylococci. Appl Environ Microbiol 55:2806–2814
    [Google Scholar]
  27. van der Mei H. C., Soct J. J., de Graaff J., Rouxhet P. G., Busscher H. J. 1991; Comparison of the physicochemical surface properties of Streptococcus rattus with those of other mutant streptococcal species. Caries Res 25:415–423
    [Google Scholar]
  28. van der Mei H. C., Cowan M. M., Genet M. J., Rouxhet P. G., Busscher H.J. 1992; Structural and physicochemical surface properties of Serratia marcescens . Can J Microbiol 38:1033–1041
    [Google Scholar]
  29. Messner P., Sleytr U. B. 1991; Bacterial surface layer glyco-proteins. Glycobiology 1:545–551
    [Google Scholar]
  30. Messner P., Sleytr U. B. 1992; Crystalline bacterial cell surface layers (S-layers). Adv Microb Physiol 33:213
    [Google Scholar]
  31. Möschl A., Schäffer G, Sleytr U. B., Messner P., Christian R., Schulz G. 1993; Characterization of the S-layer glycoproteins of two lactobacilli. Advances in Paracrystalline Surface Layers pp 281–284 Edited by Beveridge T., Koval S. NY: Plenum Press;
    [Google Scholar]
  32. Rouxhet P. G., Genet M. J. 1991; Chemical composition of the microbial cell surface by X-ray photoelectron spectroscopy. In Microbial Cell Surface Analysis: Structural and Physico-chemical Methods pp 173–220 Edited by Mozes N., Handley P. S., Busscher H. J., Rouxhet P. G. NY: VCH;
    [Google Scholar]
  33. Rouxhet P. G., Mozes N., Dengis P. B., Dufrêne Y. F., Gerin P. A., Genet M. J. 1994; Application of X-ray photoelectron spectroscopy to microorganisms. Colloids Surf B: Biointerfaces 2:347–369
    [Google Scholar]
  34. Thiery J. M. 1967; Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J Microsc (Paris) 6:987–1018
    [Google Scholar]
  35. Turner K. W., Morris H. A., Martley F. G. 1983; Swiss type cheese. II. The role of thermophilic lactobacilli in sugar fermentation. NZ J Dairy Sci Technol 18:117–123
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-141-1-11
Loading
/content/journal/micro/10.1099/00221287-141-1-11
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error