1887

Abstract

In previous experiments, the gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified. To investigate expression of the gene, S1 nuclease mapping and Northern blot hybridization were performed using RNA prepared from cultivated under various conditions. These studies suggested monocistronic organization and developmental regulation of the gene. A single promoter, -P, was identified upstream of the coding region. In cultures grown on solid medium in the absence of glucose, its transcription was induced at the time of aerial mycelium formation. In addition, transcription was also induced in substrate mycelium by glucose. A promoter-bearing DNA fragment was inserted into two promoter-probe vectors, to give expression patterns consistent with the results of direct RNA analysis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-11-3555
1997-11-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/11/mic-143-11-3555.html?itemId=/content/journal/micro/10.1099/00221287-143-11-3555&mimeType=html&fmt=ahah

References

  1. Angell S., Schwarz E., Bibb M. 1992; The glucose kinase gene of Streptomyces coelicolor A3(2): its nucleotide sequence, transcriptional analysis and role in glucose repression.. Mol Microbiol 6:2833–2844
    [Google Scholar]
  2. Ausubel F.M., Brent R., Kingston R.E., Moore D.O., Seidman J.S., Smith J.A., Struhl K. 1987 Current Protocols in Molecular Biology. New York:: Wiley.;
    [Google Scholar]
  3. Bradford M.M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.. Anal Biochem 72:248–254
    [Google Scholar]
  4. Charpentier B., Branlant C. 1994; The Escherichia coli gap Agene is transcribed by the vegetative RNA polymerase holoenzyme Eσ70 and by the heat shock RNA polymerase Eσ32.. J Bacteriol 176:830–839
    [Google Scholar]
  5. Clayton T.M., Bibb M.J. 1990; Streptomyces promoter-probe plasmids that utilise the xylE gene of Pseudomonas putida.. Nucleic Acids Res 18:1077
    [Google Scholar]
  6. Conway T., Sewell G.W., Ingram L.O. 1987; Glyceraldehyde-3-phosphate dehydrogenase gene from Zymomonas mobilis:cloning, sequencing, and identification of promoter region.. J Bacteriol 169:5653–5662
    [Google Scholar]
  7. Eikmanns B.J. 1992; Identification, sequence analysis, and expression of a Corynebacterium glutamicum gene cluster encoding the three glycolytic enzymes, glyceraldehyde 3-phosphate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomerase.. J Bacteriol 174:6076–6086
    [Google Scholar]
  8. Gallegos M.T., Michan C., Ramos J.L. 1993; The XylS/AraC family of regulators.. Nucleic Acids Res 21:807–810
    [Google Scholar]
  9. Harris J.I., Waters M. 1976; Glyceraldehyde-3-phosphate dehydrogenase.. In The Enzymes XIII pp. 1–49 Boyer P.D. Edited by New York:: Academic Press.;
    [Google Scholar]
  10. Homerová D., Benada O., Kofroňová O., Řežuchová B., Kormanec J. 1996; Disruption of a glycogen branching enzyme gene, glgB, specifically affects the sporulation-associated phase of glycogen accumulation in Streptomyces aureofaciens.. Microbiology 142:1201–1208
    [Google Scholar]
  11. Hopwood D.A., Bibb M.J., Chater K.F., Kieser T., Bruton C.J., Kieser H.M., Lydiate D.L., Smith C.P., Ward J.M., Schrempf H. 1985 Genetic Manipulation of Streptomyces. A Laboratory Manual. Norwich:: The John Innes Foundation.;
    [Google Scholar]
  12. Horinouchi S., Beppu T. 1985; Construction and application of a promoter-probe plasmid that allows chromogenic identification in Streptomyces lividans.. J Bacteriol 162:406–412
    [Google Scholar]
  13. Horinouchi S., Hara O., Beppu T. 1983; Cloning of a pleiotropic gene that positively controls biosynthesis of A-factor, actinorhodin, and prodigiosin in Streptomyces coelicolor A3 (2) and Streptomyces lividans.. J Bacteriol 155:1238–1248
    [Google Scholar]
  14. Ingram C., Brawner M., Youngman P., Westpheling J. 1989; XylE function as an efficient reporter gene in Streptomyces spp.: use for the study of galP1, a catabolite-controlled promoter.. J Bacteriol 171:6617–6624
    [Google Scholar]
  15. Kormanec J., Farkašovský M. 1993; Differential expression of principal sigma factor homologues of Streptomyces aureofacienscorrelates with the developmental stage.. Nucleic Acids Res 21:3647–3652
    [Google Scholar]
  16. Kormanec J., Farkašovský M. 1994; Isolation of total RNA? from yeast and bacteria and detection of rRNA in Northern blots.. Bio Techniques 17:838–842
    [Google Scholar]
  17. Kormanec J., Řežuchová B., Farkašovský M. 1993; Optimization of Streptomyces aureofaciens transformation and disruption of the hrdA gene encoding a homologue of the principal σ-factor.. J Gen Microbiol 139:2525–2529
    [Google Scholar]
  18. Kormanec J., Potúčková L., Řežuchová B. 1994; The Streptomyces aureofaciens homologue of whiG encoding a putative sigma factor essential for sporulation.. Gene 143:101–103
    [Google Scholar]
  19. Kormanec J., Lempelová A., Farkašovský M., Homerová D. 1995; Cloning, sequencing and expression in Escherichia coli of a Streptomyces aureofaciens gene encoding glyceraldehyde-3-phosphate dehydrogenase.. Gene 165:77–80
    [Google Scholar]
  20. Kormanec J., Homerová D., Potúčková L., Nováková R., Řežuchová B. 1996; Differential expression of two sporulation specific σ-factors of Streptomyces aureofaciens correlates with the developmental stage.. Gene 181:19–27
    [Google Scholar]
  21. Machida M., Chang Y.-C., Manabe M., Yasakawa M., Kunihiro S., Jigami Y. 1996; Molecular cloning of a cDNA encoding enolase from the filamentous fungus, Aspergillus oryzae.. Curr Genet 30:423–431
    [Google Scholar]
  22. Maxam A.M., Gilbert W. 1980; Sequencing end-labelled DNA with base specific chemical cleavages.. Methods Enzymol 65:499–560
    [Google Scholar]
  23. Pernodet J.L., Boccard F., Alegre M.T., Gagnat J., Guerineau M. 1989; Organization and nucleotide sequence analysis of a ribosomal RNA gene cluster from Streptomyces ambofaciens.. Gene 79:33–46
    [Google Scholar]
  24. Plaskitt K.A., Chater K.F. 1995; Influences of developmental genes on localized glycogen deposition in colonies of a mycelial procaryote, Streptomyces coelicolor A3(2): a possible interface between metabolism and morphogenesis.. Phil Trans R Soc Lond B 347:105–121
    [Google Scholar]
  25. Smith C.P., Chater K.F. 1988; Structure and regulation of controlling sequences for the Streptomyces coelicolor glycerol operon.. J Mol Biol 204:569–580
    [Google Scholar]
  26. Strohl W.R. 1992; Compilation and analysis of DNA sequences associated with apparent streptomycete promoters.. Nucleic Acids Res 20:961–974
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-11-3555
Loading
/content/journal/micro/10.1099/00221287-143-11-3555
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error