f A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system
- Authors: Simon C. Andrews4, Ben C. Berks, Joseph McClay, Andrew Ambler, Michael A. Quail, Paul Golby, John R. Guest
- 4Author for correspondence: Simon C. Andrews. Tel: + 44 114 222 6194. Fax: +44 114 272 8697. e-mail: email@example.com
- Microbiology, November 1997 143: 3633-3647, doi: 10.1099/00221287-143-11-3633
- Subject: Bioenergetics And Transport
- Published Online:
The nucleotide sequence has been determined for a twelve-gene operon of Escherichia coli designated the hyf operon (hyfABCDEFGHIR-focB). The hyf operon is located at 55.8-56.0 min and encodes a putative nine-subunit hydrogenase complex (hydrogenase four or Hyf), a potential formate- and σ54dependent transcriptional activator, HyfR (related to FhlA), and a possible formate transporter, FocB (related to FocA). Five of the nine Hyf-complex subunits are related to subunits of both the E. coli hydrogenase-3 complex (Hyc) and the proton-translocating NADH:quinone oxidoreductases (complex I and Nuo), whereas two Hyf subunits are related solely to NADH:quinone oxidoreductase subunits. The Hyf components include a predicted 523 residue [Ni-Fe] hydrogenase (large subunit) with an N-terminus (residues 1-170) homologous to the 30 kDa or NuoC subunit of complex I. It is proposed that Hyf, in conjunction with formate dehydrogenase H (Fdh-H), forms a hitherto unrecognized respiration-linked proton-translocating formate hydrogenlyase (FHL-2). It is likely that HyfR acts as a formate-dependent regulator of the hyf operon and that FocB provides the Hyf complex with external formate as substrate.
© Society for General Microbiology 1997 | Published by the Microbiology Society
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