@article{mbs:/content/journal/micro/10.1099/00221287-143-2-303, author = "Cormack, Brendan P. and Bertram, Gwyneth and Egerton, Mark and Gow, Neil A. R. and Falkow, Stanley and Brown, Alistair J. P.", title = "Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans", journal= "Microbiology", year = "1997", volume = "143", number = "2", pages = "303-311", doi = "https://doi.org/10.1099/00221287-143-2-303", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-2-303", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "Saccharomyces cerevisiae", keywords = "green fluorescent protein", keywords = "Candida albicans.", keywords = "enhanced GFP", abstract = "The green fluorescent protein (GFP) of Aequorea victoria has been developed here as a reporter for gene expression and protein localization in Candida albicans. When wild-type (wt) GFP was expressed in C. albicans, it was not possible to detect fluorescence or a translation product for the wt protein. Since this was probably due in part to the presence of the non-canonical CTG serine codon in the Aequorea sequence, this codon was changed to the leucine codon TTG. C. albicans cells expressing this construct contained GFP mRNA but were non-fluorescent and contained no detectable translation product. Hence a codon-optimized GFP gene was constructed in which all of the 239 amino acids are encoded by optimal codons for C albicans. In this gene were also incorporated two previously identified mutations in the chromophore that increase GFP fluorescence. C. albicans cells expressing this yeast-enhanced GFP gene (yEGFP3) are fluorescent and contain GFP protein. yEGFP3 can be used as a versatile reporter of gene expression in C. albicans and Saccharomyces cerevisiae and the optimized GFP described here should have broad applications in these and other fungal species.", }