f The topoisomerase I gene from Candida albicans
- Authors: Weidong Jiang, David Gerhold, Eric B. Kmiec, Melinda Hauser, Jeffrey M. Becker, Yigal Koltin4
- 4Author for correspondence: Yigal Koltin Tel: +1 617 374 9090 Fax:+ 1 617 225 2997 e-mail: firstname.lastname@example.org
- Microbiology, February 1997 143: 377-386, doi: 10.1099/00221287-143-2-377
- Subject: Candida Albicans
- Published Online:
We report here the cloning of the Candida albicans genomic topoisomerase I gene (TOP1) by use of PCR and subsequent hybridization. The predicted protein sequence shared 58.8% identity with the Saccharomyces cerevisiae topoisomerase I and 30-50% identity with other eukaryotic topoisomerase I proteins. A conditional gene disruption strain (CWJ477) was constructed so that one copy of TOP1 was deleted and the other copy of TOP1 was placed under a regulatable promoter. Under repressed conditions, cells grew slowly and cell morphology was abnormal. The virulence of CWJ477 was markedly reduced in a mouse model system, and that of the single gene knockout strain was slightly attenuated, indicating that TOP1 might play a role in the infection of C. albicans in mice in a dose-dependent manner. Despite the reduced virulence of both the single and double knockout strains, viable cells of the pathogen were recovered from the kidneys as late as 22 d post-infection.
© Society for General Microbiology 1997 | Published by the Microbiology Society
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