The topoisomerase I gene from Candida albicans
Jiang, Weidong and Gerhold, David and Kmiec, Eric B. and Hauser, Melinda and Becker, Jeffrey M. and Koltin, Yigal,, 143, 377-386 (1997),
doi = https://doi.org/10.1099/00221287-143-2-377,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= We report here the cloning of the Candida albicans genomic topoisomerase I gene (TOP1) by use of PCR and subsequent hybridization. The predicted protein sequence shared 58.8% identity with the Saccharomyces cerevisiae topoisomerase I and 30-50% identity with other eukaryotic topoisomerase I proteins. A conditional gene disruption strain (CWJ477) was constructed so that one copy of TOP1 was deleted and the other copy of TOP1 was placed under a regulatable promoter. Under repressed conditions, cells grew slowly and cell morphology was abnormal. The virulence of CWJ477 was markedly reduced in a mouse model system, and that of the single gene knockout strain was slightly attenuated, indicating that TOP1 might play a role in the infection of C. albicans in mice in a dose-dependent manner. Despite the reduced virulence of both the single and double knockout strains, viable cells of the pathogen were recovered from the kidneys as late as 22 d post-infection.,
language=,
type=