f Biochemical and genetic characterization of Rbf1p, a putative transcription factor of Candida albicans
- Authors: Nobuya Ishii, Mutumi Yamamoto, Fumie Yoshihara, Mikio Arisawa, Yuhko Aoki2
- 2Author for correspondence: Yuhko Aoki. Tel: +81 467 47 6773. Fax: +81 467 46 5320. e-mail: firstname.lastname@example.org
- Microbiology, February 1997 143: 429-435, doi: 10.1099/00221287-143-2-429
- Subject: Candida Albicans
- Published Online:
A Candida albicans gene encoding a novel DNA-binding protein that bound to the RPG box of Saccharomyces cerevisiae and the telomeric repeat sequence o C albicans was previously cloned and designated RBF1 (RPG-box-binding factor). In this report, determination of the functional domains of the protein is described. The DNA-binding domain was 140 aa in length, was centrally located between two glutamine-rich regions, and correlated with transcriptional activation in S. cerevisiae. The results, together with the previous finding that showed its predominant localization in the nucleus, suggest that this DNA-binding protein could be a transcription factor. Disruption of the functional RBF1 gene of C. albicans strains caused an alteration in cell morphology to the filamentous form on all solid and liquid media tested. Thus, we speculate that Rbf1p may be involved in the regulation of the transition between yeast and filamentous forms at the level of transcription.
© Society for General Microbiology 1997 | Published by the Microbiology Society
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