RT Journal Article SR Electronic(1) A1 Schmid, Roland A1 Bernhardt, Jörg A1 Antelmann, Haike A1 Völker, Andrea A1 Mach, Hiltraut A1 Völker, Uwe A1 Hecker, MichaelYR 1997 T1 Identification of vegetative proteins for a two-dimensional protein index of Bacillus subtilis JF Microbiology, VO 143 IS 3 SP 991 OP 998 DO https://doi.org/10.1099/00221287-143-3-991 PB Microbiology Society, SN 1465-2080, AB Twenty-three of the most prominent spots which are visible on two-dimensional (2-D) protein gels of Bacillus subtilis crude extracts were selected as marker spots for the construction of a 2-D protein index. N-terminal sequencing of the corresponding proteins resulted in the identification of enzymes involved in glycolysis, TCA cycle, pentose phosphate cycle, amino acid metabolism, nucleotide biosynthesis and translation. Using computer analysis of the 2-D protein gels, most of these metabolic enzymes were found to be synthesized at a reduced rate after different stresses and glucose starvation. Such an approach permits a rapid and global evaluation of the regulation of different branches of metabolism in response to various physiological conditions., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-143-3-991