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Volume 143, Issue 4

Effects of alternative methyl group acceptors on the growth energetics of the -demethylating anaerobe Free

Abstract

The anaerobic can metabolize the methyl groups of methoxylated aromatic compounds either to acetate or to dimethyl sulphide. The effects of this metabolic flexibility were investigated under conditions of excess; substrate (batch culture) and substrate limitation (chemostat culture). Growth yield data suggest that transfer of the methyl groups to sulphide, in contrast to the homoacetogenic transfer to CO, was not coupled to energy conservation. Under conditions of excess substrate, methyl groups were quantitatively transferred to sulphide. Growth yields decreased but growth rates increased upon the addition of sulphide during exponential growth in pH- and sulphide-regulated batch cultures. From the measured growth yields, the Gibbs free energy dissipation of catabolism plus anabolism ( ) was calculated using stoichiometric equations incorporating biomass formation (macrochemical equations). The observed increase in growth rate correlated well with an increase in , suggesting a relationship between growth kinetics and growth energetics. During steady-state growth in pH- and sulphide-regulated chemostat culture, a considerable fraction of the methyl groups was converted to acetate, despite the presence of sulphide. This resulted in similar growth yields and correspondingly similar values in the presence and absence of sulphide. Apparently, uncouples catabolism and anabolism in batch culture under conditions of excess substrate to a greater extent than in the chemostat under substrate limitation, by transferring the methyl groups quantitatively to sulphide and thereby dissipating the Gibbs free energy change of the methyl transfer. The physiological significance of these findings could be that adjusts the energetics of its metabolism to the growth conditions (i) to maximize the growth rate if substrate is available in excess or, (ii) to maximize the growth yield if substrate is limiting.

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Keyword(s): Gibbs free energy dissipation , Holophaga foetida , O-demethylation and sulphide methylanon, syringate
© Society for General Microbiology 1997
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1997-04-01
2024-04-20
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References

  1. Bache R., Pfennig N. 1981; Selective isolation of Acetobacterium woodii on methoxylated aromatic acids and determination of growth yields.. Arch Microbiol 130:250–261
     [Google Scholar]
  2. Bak F., Finster K., Rothfuss F. 1992; Formation of dimethylsulfide and methanethiol from methoxylated aromatic compounds and inorganic sulfide by newly isolated anaerobic bacteria.. Arch Microbiol 157:529–53
     [Google Scholar]
  3. Becher B., MUller V., Gottschalk G. 1992; N 5-methyl-tetrahydromethanopterin: coenzyme M methyltransferase of Methanosarcina strain Göl is a Na+ translocating membrane protein.. J Bacteriol 174:7656–7660
     [Google Scholar]
  4. Brune A., Schink B. 1992; Phloroglucinol pathway in the strictly anaerobic Pelobacter acidigallici: fermentation of trihydroxybenzenes to acetate via triacetic acid.. Arch Microbiol 157:417–424
     [Google Scholar]
  5. Cline J. D. 1969; Spectrometric determination of hydrogen sulfide in natural waters.. Limnol Oceanogr 14:454–458
     [Google Scholar]
  6. Cypionka H. 1986; Sulfide-controlled continuous culture of sulfate-reducing bacteria.. J Microbiol Methods 5:1–9
     [Google Scholar]
  7. van Dam K., Mulder M. M., Teixeira de Mattos M. J., Westerhoff H. V. 1988; A thermodynamic view of bacterial growth. In Physiological Models in Microbiology, vol. I, pp. 25–48. Edited by M. J. Bazin & J. I. Prosser. Boca Raton, FL: CRC Press..
    [Google Scholar]
  8. Drotar A., Fall L. R., Travernier J. E., Fall R. 1987; Enzymatic methylation of sulfide, selenite, and organic thiols by Tetrahymena thermophila.. Appl Environ Microbiol 53:2111–2118
     [Google Scholar]
  9. Finster K., Tanimoto Y., bak F. 1992; Fermentation of methanethiol and dimethylsulfide by a newly isolated methanogenic bacterium.. Arch Microbiol 157:425–430
     [Google Scholar]
  10. Frazer A. C. 1994; O-Demethylation and other transformations of aromatic compounds by acetogenic bacteria. In Acetogenesis, pp. Edited by H. L. Drake. New York, London: Chapman & Hall..
    [Google Scholar]
  11. Heijnen J. J. 1994; Thermodynamics of microbial growth and its implications for process design.. Tibtech 12:483–492
     [Google Scholar]
  12. Heijnen J. J., van Dijken J. P. 1991; In a search of a thermodynamic description of biomass yields for the chemotrophic growth of microorganisms.. Biotechnol Bioeng 39:833–858
     [Google Scholar]
  13. Kreft J.-U., Schink B. 1993; Demethylation and degradation of phenylmethylethers by the sulfide-methylating homoacetogenic bacterium strain TMBS 4.. Arch Microbiol 159:308–315
     [Google Scholar]
  14. Kreft J.-U., Schink B. 1994; O-Demethylation by the homoacetogenic anaerobe Holophaga foetida studied by a new photometric methylation assay using electrochemically produced cob(I)alamin.. Eur J Biochem 226:945–951
     [Google Scholar]
  15. Kreikenbohm R. 1984; Untersuchungen von definierten Mischkulturen anaerober Bakterien in kontinuierlicher Kultur. PhD Thesis, University of Konstanz, Germany..
    [Google Scholar]
  16. Krumböck M., Conrad R. 1991; Metabolism of position-labelled glucose in anoxic methanogenic paddy soil and lake sediment.. FEMS Microbiol Ecol 85:247–256
     [Google Scholar]
  17. Liesack W., Bak F., Kreft J.-U., Stackebrandt E. 1994; Holophaga foetida gen. nov., sp. nov., a new, homoacetogenic bacterium degrading methoxylated aromatic compounds.. Arch Microbiol 162:85–90
     [Google Scholar]
  18. Matsushita K., Ohnishi T., Kaback H. R. 1987; NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain.. Biochemistry 26:7732–7737
     [Google Scholar]
  19. Mavrovouniotis M. L. 1991; Estimation of standard Gibbs energy changes of biotransformations.. J Biol Cbem 266:14440–14445
     [Google Scholar]
  20. Neijssel O. M., Teixeira de Mattos M. J. 1994; The energetics of bacterial growth: a reassessment.. Mol Microbiol 13:179–182
     [Google Scholar]
  21. Pirt S. J. 1965; The maintenance energy of bacteria in growing cultures.. Proc R Soc Lond Ser B 163:224–231
     [Google Scholar]
  22. Rutgers M., Balk P. A., van Dam K. 1989; Effect of concentration of substrates and products on the growth of Klebsiella pneumoniae in chemostat cultures.. Biochim Biophys Acta 977:142–149
     [Google Scholar]
  23. Schink B., Pfennig N. 1982; Fermentation of trihydroxybenzenes by Pelobacter acidigallici gen. nov. sp. nov., a new strictly anaerobic, non-sporeforming bacterium.. Arch Microbiol 133:195–201
     [Google Scholar]
  24. Stucki J. W. 1980; The optimal efficiency and the economic degrees of coupling of oxidative phosphorylation.. Eur J Biochem 109:269–283
     [Google Scholar]
  25. Teixeira de Mattos M. J., Tempest D. W. 1983; Metabolic and energetic aspects of the growth of Klebsiella aerogenes NCTC 418 on glucose in anaerobic chemostat culture.. Arch Microbiol 134:80–85
     [Google Scholar]
  26. Tempest D. W., Neijssel O. M. 1984; The status of Y ATP and maintenance energy as biologically interpretable phenomena.. Annu Rev Microbiol 38:459–486
     [Google Scholar]
  27. Thauer R. K., Jungermann K., Decker K. 1977; Energy conservation in chemotrophic anaerobic bacteria.. Bacteriol Rev 41:100–180
     [Google Scholar]
  28. Tschech A., Pfennig N. 1984; Growth yield increase linked to caffeate reduction in Acetobacterium woodii. . Arch Microbiol 137:163–167
     [Google Scholar]
  29. de Vries W., Kapteijn W. M. C., van der Beek E. G., Stouthamer A. H. 1970; Molar growth yields and fermentation balances of Lactobacillus casei L3 in batch cultures and in continuous cultures.. J Gen Microbiol 63:333–345
     [Google Scholar]
  30. Westerhoff H. V., van Dam K. 1987; Thermodynamics and Control of Biological Free-energy Transduction. Amsterdam: Elsevier.
     [Google Scholar]
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Effects of alternative methyl group acceptors on the growth energetics of the O-demethylating anaerobe Holophaga foetida
Microbiology 143, 1105 (1997); https://doi.org/10.1099/00221287-143-4-1105
/content/journal/micro/10.1099/00221287-143-4-1105
/content/journal/micro/10.1099/00221287-143-4-1105
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