f Isolation and characterization of the gene encoding single-stranded-DNA-binding protein (SSB) from four marine Shewanella strains that differ in their temperature and pressure optima for growth
- Authors: Lakshmi N. Chilukuri, Douglas H. Bartlett
- Author for correspondence: Douglas H. Bartlett. Tel: +1 619 534 5233. Fax: +1 619 534 7313. e-mail: dbartlett@:ucsd.edu
- First Published Online: 01 April 1997, Microbiology 143: 1163-1174, doi: 10.1099/00221287-143-4-1163
- Subject: Biochemistry
- Issue Published:
The ssb gene, coding for single-stranded-DNA-binding protein (SSB), was cloned from four marine Shewanella strains that differed in their temperature and pressure optima and ranges of growth. All four Shewanella ssb genes complemented Escherichia coli ssb point and deletion mutants, with efficiencies that varied with temperature and ssb gene source. The Shewanella SSBs are the largest bacterial SSBs identified to date (24.9-26.3 kDa) and may be divided into conserved amino- and carboxy-terminal regions and a highly variable central region. Greater amino acid sequence homology was observed between the Shewanella SSBs as a group (72-87%) than with other bacterial SSBs (52-69%). Analysis of the amino acid composition of the Shewanella SSBs revealed several features that could correlate with pressure or temperature adaptation. SSBs from the three low-temperature-adapted Shewanella strains were an order of magnitude more hydrophilic than that from the mesophilic strain, and differences in the distribution of eight amino acids were identified which could contribute to either the temperature or pressure adaptation of the proteins. The SSBs from all four Shewanella strains were overproduced and partially purified based upon their ability to bind single-stranded DNA. The differences found among the Shewanella SSBs suggest that these proteins will provide a useful system for exploring the adaptation of protein-protein and protein-DNA interactions at low temperature and high pressure.
- Keyword(s): temperature adaptation, pressure adaptation, single-stranded-DNA-binding protein (SSB), marine shewanellas
© Society for General Microbiology 1997 | Published by the Microbiology Society
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