f Differences in genetic diversity of nonecapsulated Haemophilus influenzae from various diseases
- Authors: Loek van Alphen, Dominique A. Caugant, Birgitta Duim, Maria O'Rourke, Lucas D. Bowler†
- Author for correspondence: Loek van Alphen. Tel: +31 20 5664862. Fax: +31 20 6979271.
- First Published Online: 01 April 1997, Microbiology 143: 1423-1431, doi: 10.1099/00221287-143-4-1423
- Subject: Pathogenicity And Medical Microbiology
- Issue Published:
Genetic relationships among 80 isolates of nonencapsulated Haemophilus influenzae recovered from different disease types were determined by multilocus enzyme electrophoresis (MEE) at 13 enzyme loci in an attempt to assess the association between multilocus genotype and disease. The isolates were obtained from 15 patients with meningitis, 10 with otitis media, 19 with chronic bronchitis, 20 with cystic fibrosis, and 16 were obtained from healthy carriers. The 80 isolates were assigned to 69 electrophoretic types (ETs) falling into 5 groups. Isolates from each disease entity were represented by a variety of genotypes; however, cluster analysis from a matrix of genetic distances between ETs revealed that the ETs of the otitis media and meningitis isolates were all clustered within a genetic distance of 0.55 (group 1). In addition, no genotypes were shared between H. influenzae carrier isolates and isolates from cases of disease. H. influenzae isolates from healthy individuals were distributed significantly differently from those from chronic bronchitis meningitis and otitis media patients. The genetic diversity (H) of carrier strains was greatest, although not statistically different from that of isolates from patients with disease. It was concluded that the genetic distribution of acute disease isolates is not random over the five ET groups, although the genetic diversity within the groups is not different. The effect of bacterial persistence in the host on the genetic diversity of H. influenzae is discussed.
Present address: Dept of Molecular Microbiology, School of Biological Sciences, University of Sussex, Brighton, UK.
- Keyword(s): electrophoretic type, Haemophilus influenzae, genetic diversity, multilocus enzyme electrophoresis
© Society for General Microbiology 1997 | Published by the Microbiology Society
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