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Volume 143, Issue 6

Modulation of gene expression through chromosomal positioning in Free

Abstract

Variations in expression of the genes of the NAH7 (naphthalene biodegradation) plasmid of when placed in different chromosomal locations in have been studied by employing a collection of hybrid mini-Tn transposons bearing fusions to the promoter, along with the cognate regulatory gene Insertions of reporters in the proximity of the chromosomal origin of replication, increased accumulation of β-galactosidase Position-dependent changes in expression of the reporter product could not be associated with local variations of the supercoiling in the DNA region, as revealed by probing the chromosome with mobile elements. Such variations in β-galactosidase activity (and, therefore, the expression of catabolic genes) seemed, instead, to be linked to the increase in gene dosage associated with regions close to and not to local variations in chromosome structure. The tolerance of strains to the selection markers borne by the transposons also varied in parallel with the changes in LacZ levels. The role of chromosomal positioning as a mechanism for the outcome of adaptation phenotypes is discussed.

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Keyword(s): biodegradation , catabolic operons , gyrB , NahR and positional effects
© Society Society for General Microbiology 1997
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1997-06-01
2024-04-20
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References

  1. Bachmann B. 1990; Linkage map of Escherichra coli K-12, edition 8.. Microbiol Rev 54:130–197
     [Google Scholar]
  2. Beck E., Bremer E. 1980; Nucleotide sequence of the gene ompA for the outer membrane protein II of Escherichia coli.. Nucleic Acids Res 8:3011–3027
     [Google Scholar]
  3. Boulain J. C., Charbit A., Hofnung M. 1986; Mutagenesis by random linker insertion into the lamB gene of Escherichia coli K-12.. Mol Gen Genet 205:339–348
     [Google Scholar]
  4. Braun-Brenton C., Hofnung M. 1986; In vivo and in vitro functional alterations of the bacteriophage lambda receptor in lamB missense mutants of Escherichia coli K12.. J Bacteriol 148:845–852
     [Google Scholar]
  5. Chu G., Vollrath D., Davis R. W. 1986; Separation of large DNA molecules by contour-clamped homogeneous electric fields.. Science 234:1582–1585
     [Google Scholar]
  6. Chuang S., Daniels D. L., Blattner F. R. 1993; Global regulation of gene expression in Escherichia coli.. J Bacteriol 175:2026–2036
     [Google Scholar]
  7. Daniels D. L. 1990; The complete AvrII restriction map of the E. coli genome and comparisons of several laboratory strains.. Nucleic Acids Res 18:2649–2651
     [Google Scholar]
  8. Drlica K. 1992; Control of bacterial supercoiling.. Mol Microbiol 6:425–433
     [Google Scholar]
  9. Elena S., Cooper V. S., Lenski R. 1996; Punctuated evolution caused by selection of rare beneficial mutations.. Science 272:1802–1804
     [Google Scholar]
  10. Hayward R. S., Igarashi K., Ishihama A. 1991; Functional specialization within the α-subunit of Escherichia coli RNA polymerase.. J Mol Biol 221:23–29
     [Google Scholar]
  11. Heath J. D., Perkins J. D., Sharma B., Weinstock G. M. 1992; NotI genomic cleavage map of Escherichia coli K-12 strain MG1655.. J Bacteriol 174:558–567
     [Google Scholar]
  12. Herrero M., de Lorenzo V., Timmis K. T. 1990; Transposon vectors containing non-antibiotic selection markers for cloning and stable chromosomal insertion of foreign DNA in Gramnegative bacteria.. J Bacteriol 172:6557–6567
     [Google Scholar]
  13. Higgins N. P., Yang X., Fu Q., Roth J. 1996; Surveying a supercoil domain by using the γδ resolution system in Salmonella typhimurium.. J Bacteriol 178:2825–2835
     [Google Scholar]
  14. Liu L., Wang J. C. 1987; Supercoiling of the DNA template during transcription.. Proc Natl Acad Sci USA 84:7024–7027
     [Google Scholar]
  15. de Lorenzo V., Pérez-Martín J. 1996; Regulatory noise in prokaryotic promoters: how bacteria learn to respond to novel environmental signals.. Mol Microbiol 19:1177–1184
     [Google Scholar]
  16. de Lorenzo V., Timmis K. N. 1994; Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5- and Tn10-derived minitransposons.. Methods Enzymol 235:386–405
     [Google Scholar]
  17. de Lorenzo V., Herrero M., Jakubzik U., Timmis K. N. 1990; Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram negative eubacteria.. J Bacteriol 172:6568–6572
     [Google Scholar]
  18. de Lorenzo V., Fernández V., Herrero M., Jakubzik U., Timmis K. N. 1993; Engineering of alkyl- and gene expression with mini-transposons containing regulated promoters of biodegradative pathways of Pseudomonas.. Gene 130:41–46
     [Google Scholar]
  19. Macián F., Pérez-Roger I., Armengod M. E. 1993; An improved vector system for constructing lacZ fusions: analysis of regulation of the dnaA, dnaN, recF and gyrB genes of Escherichia coli.. Gene 145:17–24
     [Google Scholar]
  20. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory..
    [Google Scholar]
  21. van der Meer J. R., de Vos W., Harayama S., Zehnder A. 1992; Molecular mechanisms of bacterial adaptation to xenobiotic compounds.. Microbiol Rev 56:677–694
     [Google Scholar]
  22. Menzel R., Gellert M. 1983; Regulation of the genes for E. coli DNA gyrase: homeostatic control of DNA supercoiling.. Cell 34:105–113
     [Google Scholar]
  23. Miller J. H. 1972; Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory..
    [Google Scholar]
  24. Miller V. L., Mekalanos J. 1988; A novel suicide vector and its use in construction of insertion mutations : osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR.. J Bacteriol 170:2575–2583
     [Google Scholar]
  25. Miller W. G., Simons R. 1993; Chromosomal supercoiling in Escherichia coli.. Mol Microbiol 10:675–684
     [Google Scholar]
  26. Nakamura K., Inouye M. 1979; DNA sequence of the gene for the outer membrane lipoprotein of E. coli: an extremely AT-rich promoter.. Cell 18:1109–1117
     [Google Scholar]
  27. Nash H. A. 1996; The E. coli HU and IHF proteins: accessory factors for complex protein-DNA assemblies. In Regulation of Gene Expression in Escherichia coli, Edited by E. C. C. Lin & A. S. Lynch. Bethesda, MD: RG Landes Co.. 149–179
     [Google Scholar]
  28. Negri M. C., Morosini M. I., Loza E., Baquero F. 1994; In vitro selective antibiotic concentrations of β-lactams for penicillin resistant Streptococcus pneumoniae populations.. Antimicrob Agents Chemother 38:122–125
     [Google Scholar]
  29. Ohki M., Smith C. L. 1989; Tracking bacterial replication forks in vivo by pulsed field gel electrophoresis.. Nucleic Acids Res 17:3479–3490
     [Google Scholar]
  30. Pavitt G. D., Higgins C. F. 1993; Chromosomal domains of supercoiling in Salmonella typhimurium.. Mol Microbiol 10:685–696
     [Google Scholar]
  31. Plasterk R. H., Vrieling H., Van de Putte P. 1983; Transcription initiation of Mu mom depends on niethylation of the promoter region and a phage-coded transactivator.. Nature 301:344–347
     [Google Scholar]
  32. Perkins J. D., Heath J., Sharma B. R., Weinstock G. M. 1993; XbaI and BlnI genomic cleavage maps of Escherichia coli K-12 strain MG1655 and comparative analysis of other strains.. J Mol Biol 232:419–445
     [Google Scholar]
  33. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular Cloning: a Laboratory Manual. 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory..
    [Google Scholar]
  34. Schmid M., Roth J. 1987; Gene location affects expression level in Salmonella typhimurium.. J Bacteriol 169:2872–2875
     [Google Scholar]
  35. Smith C. L., Econome J., Schutt A., Klco S., Cantor C. R. 1987; A physical map of the Escherichia coli K-12 genome.. Science 236:1448–1453
     [Google Scholar]
  36. Washington J. A., Sutter V. 1980; Dilution susceptibility test: agar and macro-broth dilution procedures. In Manual of Clinical Microbiology, 3rd edn, pp. Edited by R. H. Lennette, A. Balows, W. J. Hausler, Jr & J. P. Truant. Washington, DC: American Society for Microbiology.. 453–458
     [Google Scholar]
  37. Wee S., Neilands J., Bittner M., Hemming B., Haymore B., Seetharam R. 1988; Expression, isolation and properties of Fur (ferric uptake regulation) protein of Escherichia coli K-12.. Biol Metals 1:62–68
     [Google Scholar]
  38. Williams N., Fox D., Shea C., Roseman S. 1986; Pel, the protein that permits λ DNA penetration of Escherichia coli, is encoded by a gene in ptsM and is required for mannose utilization by the phosphotransferase system.. Proc Natl Acad Sci USA 83:8934–8938
     [Google Scholar]
  39. Wong K. K., McClelland M. 1992; Dissection of the Salmonella typhimurium genome by use of a Tn5 derivative carrying rare restriction sites.. J Bacteriol 174:3807–3811
     [Google Scholar]
  40. Worcel A., Burgi E. 1972; On the structure of the folded chromosome of Escherichia coli.. J Mol Biol 71:127–147
     [Google Scholar]
  41. Wyndham R. C., Cashore A. E., Nakatsu C. H., Peel M. 1994; Catabolic transposons.. Biodegradation 5:323–342
     [Google Scholar]
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Modulation of gene expression through chromosomal positioning in Escherichia coli
Microbiology 143, 2071 (1997); https://doi.org/10.1099/00221287-143-6-2071
/content/journal/micro/10.1099/00221287-143-6-2071
/content/journal/micro/10.1099/00221287-143-6-2071
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