1887

Abstract

Summary: Trehalase activity is markedly enhanced upon addition of glucose and a nitrogen source to cells of the fission yeast This increase corresponds to a post-translational activation of the enzyme, which is controlled by cAMP-dependent and cAMP-independent pathways. Recent work has shown that overexpression of SCK1 in Schiz. pombe is able to suppress mutations that result in reduced Pka1 (cAMP-dependent protein kinase A activity, suggesting that Sck1 (suppressor of loss of cAMP-dependent protein kinase) might be a functional analogue of Pka1 in the fission yeast. Here, an analysis of the possible role of Sck1 in the activation of trehalase triggered by glucose and a nitrogen source is reported in cells that were deficient in either Pka1, Sck1 or both protein kinases. The results showed that, except in repressed cells, Sck1 probably mediates a cAMP-independent activation of trehalase following the signal(s) triggered by glucose and the nitrogen source. The absence of functional Sck1 in derepressed cells renders trehalase insensitive to activation by glucose and the nitrogen source even in the presence of Pka1, indicating that the Sck1-dependent, cAMP-independent pathway is the main signalling pathway controlling trehalase activation under derepression conditions. It is proposed that, during the activation of trehalase induced by glucose or a nitrogen source, the cAMP-Pka1 activation pathway previously characterized is to some extent parallel to this newly described one which includes Sck1 as ohosphorylating enzyme. Neither of these two pathways, however, plays a key role in the heat-induced increase in trehalase activity.

Funding
This study was supported by the:
  • DGICYT, Spain (Award PB941151)
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1997-07-01
2024-03-28
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References

  1. Byrne S. M., Hoffman C. S. 1993; Six git genes encode a glucose-induced adenylate cyclase activation pathway in the fission yeast Schizosaccharomyces pombe . J Cell Sci 105:1095–1100
    [Google Scholar]
  2. Carrillo D., Vicente-Soler J., Gacto M. 1992; Activation of neutral trehalase by fermentable sugars and cAMP in the fission yeast Schizosaccharomyces pombe . FEMS Microbiol Lett 98:61–66
    [Google Scholar]
  3. Carrillo D., Vicente-Soler J., Gacto M. 1994; Cyclic AMP signalling pathway and trehalase activation in the fission yeast Schizosaccharomyces pombe . Microbiology 140:1467–1472
    [Google Scholar]
  4. Cherry J. R., Johnson T. R., Dollard C., Shuster J. R., Denis C. L. 1989; Cyclic AMP-dependent protein kinase phosphorylates and inactivates the yeast transcriptional activator ADR1. Cell 56:409–419
    [Google Scholar]
  5. De Virgilio C., Simmen U., Hottiger T., Boller T., Wienkem A. 1990; Heat shock induces enzymes of trehalose metabolism, trehalose accumulation, and thermotolerance in Schizosaccharomyces pombe, even in the presence of cycloheximide. FEBS Lett 273:107–110
    [Google Scholar]
  6. Fernandez J., Soto T., Vicente-Soler J., Cansado J., Gacto M. 1997; Heat shock response in Schizosaccharomyces pombe cells lacking cyclic AMP-dependent phosphorylation. Curr Genet 31:112–118
    [Google Scholar]
  7. Hoffman C. S., Winston F. 1990; Isolation and characterization of mutants constitutive for expression of the fbp1 gene of Schizosaccharomyces pombe . Genetics 124:807–816
    [Google Scholar]
  8. Hoffman C. S., Winston F. 1991; Glucose repression of transcription of the Schizosaccharomyces pombe fbp1 gene occurs by a cAMP-signalling pathway. Genes Dev 5:561–571
    [Google Scholar]
  9. Holzer H. 1976; Catabolite inactivation in yeast. Trends Biochem Sci 1:178–181
    [Google Scholar]
  10. Jin M., Fujita M., Culley B. M., Apolinario E., Yamamoto M., Maundrell K., Hoffman C. S. 1995; Sck1, a high copy number suppressor of defects in the cAMP-dependent protein kinase pathway in fission yeast, encodes a protein homologous to the Saccharomyces cerevisiae SCH9 kinase. Genetics 140:457–467
    [Google Scholar]
  11. Maeda T., Watanabe Y., Kunitomo H., Yamamoto M. 1994; Cloning of the Pka1 gene encoding the catalytic subunit of the cAMP-dependent protein kinase in Schizosaccharomyces pombe . J Biol Chem 269:9632–9637
    [Google Scholar]
  12. Matsumoto K., Uno I., Oshima Y., Ishikawa T. 1982; Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase. Proc Natl Acad Sci USA 79:2355–2359
    [Google Scholar]
  13. Mbonyi K., Beullens M., Detremerie, ढ., Geerts L., Thevelein J. M. 1988; Requirement of one functional RAS gene and inability of an oncogenic RAS variant to mediate the glucose-induced cyclic AMP signal in the yeast Saccharomyces cerevisiae . Mol Cell Biol 8:3051–3057
    [Google Scholar]
  14. Munder T., Küntzel H. 1989; Glucose-induced cAMP signalling in Saccharomyces cerevisiae is mediated by CDC25 protein. FEBS Lett 242:341–345
    [Google Scholar]
  15. Soto T., Fernandez J., Vicente-Soler J., Cansado J., Gacto M. 1995a; Activation of neutral trehalase by glucose and nitrogen sources in Schizosaccharomyces pombe strains deficient in cAMP-dependent protein kinase activity. FEBS Lett 367:263–266
    [Google Scholar]
  16. Soto T., Fernandez J., Cansado J., Vicente-Soler J., Gacto M. 1995b; Glucose-induced, cyclic-AMP-independent signalling pathway for activation of neutral trehalase in the fission yeast Schizosaccharomyces pombe . Microbiology 141:2665–2671
    [Google Scholar]
  17. Soto T., Fernandez J., Vicente-Soler J., Cansado J., Gacto M. 1995c; Nitrogen-source-induced activation of neutral trehalase in Schizosaccharomyces pombe and Pachysolen tannophilus: role of cAMP as second messenger. FEMS Microbiol Lett 132:229–232
    [Google Scholar]
  18. Stettler S., Warbrick E., Prochnik S., Mackie S., Fantes P. 1996; The wis1 signal transduction pathway is required for expression of cAMP-repressed genes in fission yeast. J Cell Sci 109:1927–1935
    [Google Scholar]
  19. Thevelein J. M. 1988; Regulation of trehalase activity by phosphorylation–dephosphorylation during developmental transition in fungi. Exp Mycol 12:1–12
    [Google Scholar]
  20. Thevelein J. M. 1992; The RAS-adenylate cyclase pathway and cell cycle control in Saccharomyces cerevisiae . Antonie Leeuwenhoek 62:109–130
    [Google Scholar]
  21. Uno I., Matsumoto K., Adachi K., Ishikawa T. 1983; Genetic and biochemical evidence that trehalase is a substrate of cAMP-dependent protein kinase in yeast. J Biol Chem 258:10867–10872
    [Google Scholar]
  22. Watanabe Y., Yamamoto M. 1996; Schizosaccharomyces pombe pcr1+ encodes a CREB/ATF protein involved in regulation of gene expression for sexual development. Mol Cell Biol 16:704–711
    [Google Scholar]
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