1887

Abstract

Summary: When plasma membranes prepared from the yeast were exposed to near-UV radiation, photodecomposition of ergosterol and reduction of ATPase activity occurred simultaneously. The for ATPase activity decreased markedly with increasing near-UV dosage while the value remained constant. When ATPase solubilized from the plasma membrane was exposed to near-UV, the activity remained constant irrespective of dosage, indicating that the ATPase molecule itself was not damaged by near-UV irradiation. The relationship between content of ergosterol and ATPase activity was examined using liposomes constructed with lipids extracted from the membrane. Maximum activity of ATPase was seen at 5% ergosterol in liposomes; this activity was 2.5 times greater than that in liposomes without ergosterol. Activity of ATPase bound to liposomes with 5% ergosterol was reduced after near-UV irradiation, while the activity remained unchanged in the case of the liposomes without ergosterol. Fluidity of the liposomes with 5% ergosterol also decreased with increasing near-UV dosage. Dosage-response curves for reduction of ATPase activity and for decrease in fluidity were similar to that for photodecomposition of ergosterol. These results suggested that the reduction of ATPase activity in the membrane by near-UV irradiation was not caused by photochemical degradation of the primary structure of the ATPase molecule, but was attributable to conformational change resulting from an alteration in the higher-order structure of the membrane due to photochemical decomposition of ergosterol.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-7-2465
1997-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/7/mic-143-7-2465.html?itemId=/content/journal/micro/10.1099/00221287-143-7-2465&mimeType=html&fmt=ahah

References

  1. Allen C. F., Good P. 1971; Acyl lipids in photosynthetic systems. Methods Enzymol 123:523–547
    [Google Scholar]
  2. Anderson V. C., Thompson D. H. 1992; Triggered release of hydrophilic agents from plasmalogen liposomes using visible light or acid. Biochim Biophys Acta 1109:33–42
    [Google Scholar]
  3. Arami S., Hada M., Itadani A., Yamashita S., Hachiya K., Kanayama M., Tada M. 1993; Damage of membrane function in the yeast Saccharomyces cerevisiae by near-UV irradiation. Sci Rep Fac Agric Okayama Univ 82:1–7
    [Google Scholar]
  4. Arami S., Hada M., Tada M. 1997; Near-UV-induced absorbance change and photochemical decomposition of ergosterol in the plasma membrane of the yeast Saccharomyces cerevisiae . Microbiology 143:1665–1671
    [Google Scholar]
  5. Arnezeder C., Koliander W., Hampel W. A. 1989; Rapid determination of ergosterol in yeast cells. Anal Chim Acta 225:129–136
    [Google Scholar]
  6. Baba A., Lee E., Ohta A., Tatsuno T., Iwata H. 1981; Activation of adenylate cyclase of rat brain by lipid peroxidation. J Biol Chem 256:3679–3684
    [Google Scholar]
  7. Bose B., Agarwal S., Chatterjee S. N. 1989; UV-A induced lipid peroxidation in liposomal membrane. Radiat Environ Biophys 28:59–65
    [Google Scholar]
  8. Bose B., Agarwal S., Chatterjee S. N. 1990; Membrane lipid peroxidation by near-UV: mechanism and implications. Bio-technol Appl Biochem 12:557–561
    [Google Scholar]
  9. Braganza L., F” Blott B. H., Coe T. J., Melville D. 1983; Dye permeability at phase transitions in single and binary component phospholipid bilayers. Biochim Biophys Acta 731:137–144
    [Google Scholar]
  10. Cerbon J., Calderon V. 1991; Changes of the compositional asymmetry of phospholipids associated to the increment in the membrane surface potential. Biochim Biophys Acta 1067:139–144
    [Google Scholar]
  11. Corvera E., Mouritsen O. G., Singer M. A., Zuckermann M. J. 1992; The permeability and the effect of acyl-chain length for phospholipid bilayers containing cholesterol: theory and experiment. Biochim Biophys Acta 1107:261–270
    [Google Scholar]
  12. Darszon A., Vandenberg C. A., Schonfeld M., Ellisman M. H., Spitzer N. C., Montal M. 1980; Reassembly of protein-lipid complexes into large bilayer vesicles: perspective for membrane reconstitution. Proc Natl Acad Sci USA 77:239–243
    [Google Scholar]
  13. Davis C. B., Hammes G. G. 1989; Topology of the yeast plasma membrane proton-translocating ATPase. 1 Biol Chem 264:370–374
    [Google Scholar]
  14. Drissen A. J. M., Zheng T., In’t Veld G., Op den Kamp J. A. F., Konings W. N. 1988; Lipid requirement of the branched-chain amino acid transport system of Streptococcus cremoris . Biochemistry 27:865–872
    [Google Scholar]
  15. Fiske C. H., Subbarow Y. 1925; The colorimetric determination of phosphorus. J Biol Chem 66:375–400
    [Google Scholar]
  16. George R., McElhaney R. N. 1992; The effect of cholesterol and epicholesterol on the activity and temperature dependence of the purified, phospholipid-reconstituted (Na+ + Mg2+)-ATPase from Acholeplasma laidlawii B membranes. Biochim Biophys Acta 1107:111–118
    [Google Scholar]
  17. Hu M.-L., Tappel A. L. 1992; Potentiation of oxidative damage to proteins by ultraviolet-A and protection by antioxidants. Photochem Photobiol 56:357–363
    [Google Scholar]
  18. In’t Veld G., Drissen A. J. M., Op den Kamp J. A. F., Konings W. N. 1991; Hydrophobic membrane thickness and lipid– protein interactions of the leucine transport system of Lacto-coccus lactis . Biochim Biophys Acta 1065:203–212
    [Google Scholar]
  19. In’t Veld G., Drissen A. J. M., Konings W. N. 1992; Effect of the unsaturation of phospholipid acyl chains on leucine transport of Lactococcus lactis and membrane permeability. Biochim Biophys Acta 1108:31–39
    [Google Scholar]
  20. Ito A., Ito T. 1983; Possible involvement of membrane damage in the inactivation by broad-band near-UV radiation in Saccharomyces cerevisiae cells. Photochem Photobiol 37:395–401
    [Google Scholar]
  21. Ito T., Ito A. 1984; Enhancement of porphyrin-photo-sensitization of yeast cells by ethanol. Photochem Photobiol 40:429–434
    [Google Scholar]
  22. Kasamo K. 1986; Purification and properties of the plasma membrane H+-translocating adenosine triphosphatase of Phaseo-lus mungo L. Plant Physiol 80:818–824
    [Google Scholar]
  23. Kasamo K. 1990; Mechanism for the activation of plasma membrane H+-ATPase from rice (Oryza sativa L.) culture cells by molecular species of a phospholipid. Plant Physiol 93:1049–1052
    [Google Scholar]
  24. Kasamo K., 8t Nouchi I. 1987; The role of phospholipids in plasma membrane ATPase activity in Vigna radiata L. (Mung bean) roots and hypocotyls. Plant Physiol 83:323–328
    [Google Scholar]
  25. Kitagawa S., Takegaki M. 1992; Transbilayer incorporation of 1-pyrene-butyltrimethylammonium by blood platelets and its application for analyzing changes in physico-chemical properties of the membrane lipid bilayer induced by platelet activation. Biochim Biophys Acta 1107:231–237
    [Google Scholar]
  26. Kukreja R. C., Okabe E., Schrier G. M., Hess M. L. 1988; Oxygen radical-mediated lipid peroxidation and inhibition of Ca2+-ATPase activity of cardiac sarcoplasmic reticulum. Arch Biochim Biophys 261:447–457
    [Google Scholar]
  27. Leber R., Zinser E., Hrastnik C., Paltauf F., Daum G. 1995; Export of steryl esters from lipid particles and release of free sterols in the yeast, Saccharomyces cerevisiae . Biochim Biophys Acta 1234:119–126
    [Google Scholar]
  28. Marshanskii V. K., Novgorodov S. A., Yaguzhinskii L. S. 1983; The role of lipid peroxidation in the induction of cation transport in rat liver mitochondria. The antioxidant effect of oligomycin and dicyclohexyl carbodiimide. FEBS Lett 158:27–30
    [Google Scholar]
  29. Ohta A., Mohri T., Ohyashiki T. 1989; Effect of peroxidation on membrane-bound Ca2+-ATPase activity of the intestinal brush-border membranes. Biochim Biophys Acta 984:151–157
    [Google Scholar]
  30. Prasad R. 1985; Lipids in the structure and function of yeast membrane. Adv Lipid Res 21:187–242
    [Google Scholar]
  31. Scherer N. M., Deamer D. W. 1986; Oxidative stress impairs the function of sarcoplasmic reticulum by oxidation of sulfhydryl groups in the Ca2+-ATPase. Arch Biochem Biophys 246:589–601
    [Google Scholar]
  32. Serrano R., Montesinos C., Sanchez J. 1988; Lipid requirements of the plasma membrane ATPase from oat roots and yeast. Plant Sci 56:117–122
    [Google Scholar]
  33. Singer S. J., 8t Nicolson G. L. 1972; The fluid mosaic model of the structure of cell membranes. Science 175:720–731
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-7-2465
Loading
/content/journal/micro/10.1099/00221287-143-7-2465
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error