f Altered adherence properties of a Streptococcus gordonii hppA (oligopeptide permease) mutant result from transcriptional effects on cshA adhesin gene expression
- Authors: Roderick McNab†, Howard F. Jenkinson‡
- Author for correspondence: Howard F. Jenkinson. Tel: + 44 117 928 4304. Fax: +44 117 928 4428. e-mail: firstname.lastname@example.org
- Microbiology, January 1998 144: 127-136, doi: 10.1099/00221287-144-1-127
- Subject: Genetics And Molecular Biology
- Published Online:
Summary: Cell-surface polypeptide CshA (259 kDa) mediates multiple adherence interactions of Streptococcus gordonii. By generating a chromosomal cshA promoter (p-cshA)-cat gene fusion and measuring both CAT enzyme activity and cat mRNA levels, it was shown that cshA is expressed maximally in cells in the late exponential phase of growth in batch culture. The expression of CAT enzyme activity from the p-cshA-cat promoter fusion was 28% decreased in early stationary phase cell extracts of mutant strain OB528 in which the hppA (oligopeptide-binding lipoprotein) gene was insertionally inactivated. This effect was correlated with proportionally reduced cell-surface expression of CshA protein and with impaired adherence of hppA mutant cells to cells of an oral Actinomyces naeslundii strain. cshA promoter activity was enhanced in streptococcal cells that were incubated in conditioned culture medium as opposed to fresh medium, but this did not occur in an hppA genetic background. It is suggested that HppA is necessary for the response of cells to an extracellular factor that modulates cshA transcription, and hence affects cell-surface CshA expression and streptococcal cell adherence properties.
Present address: Department of Microbiology, Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK.
Present address: Department of Oral and Dental Science, University of Bristol, Dental Hospital and School, Lower Maudlin Street, Bristol BS1 2LY, UK.
- Keyword(s): cell-surface proteins, streptococcal cell adhesion, promoter activity, Streptococcus gordonii, oligopeptide permease
© Society for General Microbiology 1998 | Published by the Microbiology Society
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