1887

Microbiology Society logo

Volume 144, Issue 1

Altered adherence properties of a Streptococcus gordonii hppA (oligopeptide permease) mutant result from transcriptional effects on cshA adhesin gene expression Free

Abstract

Summary: Cell-surface polypeptide CshA (259 kDa) mediates multiple adherence interactions of By generating a chromosomal promoter ( gene fusion and measuring both CAT enzyme activity and mRNA levels, it was shown that is expressed maximally in cells in the late exponential phase of growth in batch culture. The expression of CAT enzyme activity from the promoter fusion was 28% decreased in early stationary phase cell extracts of mutant strain OB528 in which the (oligopeptide-binding lipoprotein) gene was insertionally inactivated. This effect was correlated with proportionally reduced cell-surface expression of CshA protein and with impaired adherence of mutant cells to cells of an oral strain. promoter activity was enhanced in streptococcal cells that were incubated in conditioned culture medium as opposed to fresh medium, but this did not occur in an genetic background. It is suggested that HppA is necessary for the response of cells to an extracellular factor that modulates transcription, and hence affects cell-surface CshA expression and streptococcal cell adherence properties.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): cell-surface proteins , oligopeptide permease , promoter activity , streptococcal cell adhesion and Streptococcus gordonii
© Society for General Microbiology 1998
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-1-127
1998-01-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/1/mic-144-1-127.html?itemId=/content/journal/micro/10.1099/00221287-144-1-127&mimeType=html&fmt=ahah

References

  1. Alloing G., de Philip P., Claverys J. -P. 1994; Three highly homologous membrane-bound lipoproteins participate in oligopeptide transport by the ami system of gram-positive Streptococcus pneumoniae. . J Mol Biol 241:44–58
     [Google Scholar]
  2. Alloing G., Granadel C., Morrison D. A., Claverys J. -P. 1996; Competence pheromone, oligopeptide permease, and induction of competence in Streptococcus pneumoniae. . Mol Microbiol 21:471–178
     [Google Scholar]
  3. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410
     [Google Scholar]
  4. Boyer H. W., Roulland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA in Escherichia coli. . J Mol Biol 41:459–472
     [Google Scholar]
  5. Burnette-Curley D., Wells V., Viscount H., Munro C., L, Fenno J. C., Fives-Taylor P., Macrina F. L. 1995; FimA, a major virulence factor associated with Streptococcus parasanguis endocarditis. Infect Immun 63:4669–4674
     [Google Scholar]
  6. Cámara M., Boulnois G. J., Andrew P. W., Mitchell T. J. 1994; A neuraminidase from Streptococcus pneumoniae has the features of a surface protein. Infect Immun 62:3688–3695
     [Google Scholar]
  7. Church G. M., Gilbert W. 1984; Genomic sequencing. Proc Natl Acad Sci USA 81:1991–1995
     [Google Scholar]
  8. Cundell D. R., Pearce B. J., Sandros J., Naughton A. M., Masure H. R. 1995; Peptide permeases from Streptococcus pneumoniae affect adherence to eukaryotic cells. Infect Immun 63:2493–2498
     [Google Scholar]
  9. Demuth D. R., Duan Y., Jenkinson H. F., McNab R., Gil S., Lamont R. J. 1997; Interruption of the Streptococcus gordonii M5 sspA/sspB intergenic region by an insertion sequence related to IS1167 of Streptococcus pneumoniae. . Microbiology 143:2047–2055
     [Google Scholar]
  10. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
     [Google Scholar]
  11. Frandsen E. V. G., Pedrazzoli V., Kilian M. 1991; Ecology of viridans streptococci in the oral cavity and pharynx. Oral Microbiol Immunol 6:129–133
     [Google Scholar]
  12. Ganeshkumar N., Hannam P. M., Kolenbrander P. E., McBride B. C. 1991; Nucleotide sequence of a gene coding for a saliva-binding protein (SsaB) from Streptococcus sanguis 12 and possible role of the protein in coaggregation with actinomyces. Infect Immun 59:1093–1099
     [Google Scholar]
  13. Haisman R. J., Jenkinson H. F. 1991; Mutants of Streptococcus gordonii Challis overproducing glucosyltransferase. J Gen Microbiol 137:483–489
     [Google Scholar]
  14. Håvarstein L. S., Gaustad P., Nes I. F., Morrison D. A. 1996; Identification of the streptococcal competence pheromone receptor. Mol Microbiol 21:863–869
     [Google Scholar]
  15. Hawley D. K., McClure W. R. 1983; Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res 11:2237–2255
     [Google Scholar]
  16. Higgins C. F. 1992; ABC transporters: from microorganisms to man. Annu Rev Cell Biol 8:67–113
     [Google Scholar]
  17. Holmes A. R., Gopal P. K., Jenkinson H. F. 1995; Adherence of Candida albicans to a cell surface polysaccharide receptor on Streptococcus gordonii. . Infect Immun 63:1827–1834
     [Google Scholar]
  18. Holmes A. R., McNab R., Jenkinson H. F. 1996; Candida albicans binding to the oral bacterium Streptococcus gordonii involves multiple adhesin-receptor interactions. Infect Immun 64:4680–4685
     [Google Scholar]
  19. Hudson M. C., Curtiss R. III 1990; Regulation of expression of Streptococcus mutans genes important to virulence. Infect Immun 58:464–470
     [Google Scholar]
  20. Hudson M. C., Stewart G. C. 1986; Differential utilization of Staphylococcus aureus promoter sequences by Escherichia coli and Bacillus subtilis. . Gene 48:93–100
     [Google Scholar]
  21. Jenkinson H. F. 1987; Novobiocin-resistant mutants of Streptococcus sanguis with reduced cell hydrophobicity and defective in coaggregation. J Gen Microbiol 133:1909–1918
     [Google Scholar]
  22. Jenkinson H. F., Demuth D. R. 1997; Structure, function and immunogenicity of streptococcal antigen I/II polypeptides. Mol Microbiol 23:183–190
     [Google Scholar]
  23. Jenkinson H. F., Easingwood R. A. 1990; Insertional inactivation of the gene encoding a 76-kilodalton cell-surface polypeptide in Streptococcus gordonii Challis has a pleiotropic effect on cell surface composition and properties. Infect Immun 58:3689–3697
     [Google Scholar]
  24. Jenkinson H. F., Lamont R. J. 1997; Streptococcal adhesion and colonization. Crit Rev Oral Biol Med 8:175–200
     [Google Scholar]
  25. Jenkinson H. F., Terry S. D., McNab R., Tannock G. W. 1993; Inactivation of the gene encoding surface protein SspA in Streptococcus gordonii DL1 affects cell interactions with human salivary agglutinin and oral actinomyces. Infect Immun 61:3199–3208
     [Google Scholar]
  26. Jenkinson H. F., Baker R. A., Tannock G. W. 1996; A binding-lipoprotein-dependent oligopeptide transport system in Streptococcus gordonii essential for uptake of hexa- and heptapeptides. J Bacteriol 178:68–77
     [Google Scholar]
  27. Ji G., Beavis R. C., Novick R. P. 1995; Cell density control of staphylococcal virulence mediated by an octapeptide pheromone. Proc Natl Acad Sci USA 92:12055–12059
     [Google Scholar]
  28. Kehoe M. A. 1994; Cell-wall-associated proteins in gram-positive bacteria. In Bacterial Cell Wall pp 217–261 Edited by Ghuysen J. -M., Hakenbeck R. London: Elsevier;
     [Google Scholar]
  29. Kolenbrander P. E., Andersen R. N., Ganeshkumar N. 1994; Nucleotide sequence of the Streptococcus gordonii PK488 co-aggregation adhesin gene, scaA, and ATP-binding cassette. Infect Immun 62:4469–4480
     [Google Scholar]
  30. Kunji E. R. S., Mierau I., Hagting A., Poolman B., Konings W. N. 1996; The proteolytic systems of lactic acid bacteria. Antonie Leeuwenhoek 70:187–221
     [Google Scholar]
  31. Lebrun M., Mengaud J., Ohayon H., Nato F., Cossart P. 1996; Internalin must be on the bacterial surface to mediate entry of Listeria monocytogenes into epithelial cells. Mol Microbiol 21:579–592
     [Google Scholar]
  32. Leonard B. A. B., Podbielski A., Hedberg P. J., Dunny G. M. 1996; Enterococcus faecalis pheromone binding protein, PrgZ, recruits a chromosomal oligopeptide permease system to import sex pheromone cCF10 for induction of conjugation. Proc Natl Acad Sci USA 93:260–264
     [Google Scholar]
  33. Lunsford R. D. 1995; Recovery of RNA from oral streptococci. Bio Techniques 18:412–413
     [Google Scholar]
  34. McNab R., Jenkinson H. F., Loach D. M., Tannock G. W. 1994; Cell-surface-associated polypeptides CshA and CshB of high molecular mass are colonization determinants in the oral bacterium Streptococcus gordonii. . Mol Microbiol 14:743–754
     [Google Scholar]
  35. McNab R., Holmes A. R., Jenkinson H. F. 1995; Cell-surface polypeptides as determinants of hydrophobicity in Streptococcus gordonii and Streptococcus sanguis. . Colloids Surf 5:135–142
     [Google Scholar]
  36. McNab R., Holmes A. R., Clarke J. M., Tannock G. W., Jenkinson H. F. 1996; Cell surface polypeptide CshA mediates binding of Streptococcus gordonii to other oral bacteria and to immobilized fibronectin. Infect Immun 64:4204–4210
     [Google Scholar]
  37. Pakula R., Walczak W. 1963; On the nature of competence of transformable streptococci. J Gen Microbiol 31:125–133
     [Google Scholar]
  38. Patti J. M., Allen B. L., McGavin M. J., Höök M. 1994; MSCRAMM-mediated adherence of microorganisms to host tissues. Annu Rev Microbiol 48:585–617
     [Google Scholar]
  39. Pearce B. J., Naughton A. M., Masure H. R. 1994; Peptide permeases modulate transformation in Streptococcus pneumoniae. . Mol Microbiol 12:881–892
     [Google Scholar]
  40. Podbielski A., Schnitzler N., Beyhs P., Boyle M. D. P. 1996a; M-related protein (Mrp) contributes to group A streptococcal resistance to phagocytosis by human granulocytes. Mol Microbiol 19:429–441
     [Google Scholar]
  41. Podbielski A., Pohl B., Woischnik M., Körner C., Schmidt K. -H., Rozdzinski E., Leonard B. A. B. 1996b; Molecular characterization of group A streptococcal (GAS) oligopeptide permease (Opp) and its effect on cysteine protease production. Mol Microbiol 21:1087–1099
     [Google Scholar]
  42. Sabelnikov A. G., Greenberg B., Lacks S. A. 1995; An extended −10 promoter alone directs transcription of the DpnII operon of Streptococcus pneumoniae. . J Mol Biol 250:144–155
     [Google Scholar]
  43. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual 2nd edn Cold Spring Harbor; NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  44. Schneewind O., Fowler A., Faull K. F. 1995; Structure of the cell wall anchor of surface proteins in Staphylococcus aureus. . Science 268:103–106
     [Google Scholar]
  45. Shaw W. V. 1975; Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. Methods Enzymol 43:737–755
     [Google Scholar]
  46. Solomon J. M., Lazazzera B. A., Grossman A. D. 1996; Purification and characterization of an extracellular peptide factor that affects two different developmental pathways in Bacillus subtilis. . Genes Dev 10:2014–2024
     [Google Scholar]
  47. Sutcliffe I. C., Russell R. R. B. 1995; Lipoproteins of gram-positive bacteria. J Bacteriol 177:1123–1128
     [Google Scholar]
  48. Tam R., Saier M. H. Jr 1993; Structural, functional, and evolutionary relationships among extracellular solute-binding receptors of bacteria. Microbiol Rev 57:320–346
     [Google Scholar]
  49. Tao L., LeBlanc D. J., Ferretti J. J. 1992; Novel streptococcal-integration shuttle vectors for gene cloning and inactivation. Gene 120:105–110
     [Google Scholar]
  50. Tao L., Sutcliffe I. C., Russell R. R. B., Ferretti J. J. 1993; Transport of sugars, including sucrose, by the msm system of Streptococcus mutans. . J Dent Res 72:1386–1390
     [Google Scholar]
  51. de Vos W. M., Kuipers O. P., van der Meer J. R., Siezen R. J. 1995; Maturation pathway of nisin and other lantibiotics: post-translationally modified antimicrobial peptides exported by gram-positive bacteria. Mol Microbiol 17:427–437
     [Google Scholar]
  52. Whittaker C. J., Klier C. M., Kolenbrander P. E. 1996; Mechanisms of adhesion by oral bacteria. Annu Rev Microbiol 50:513–552
     [Google Scholar]
  53. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-1-127
Loading
Altered adherence properties of a Streptococcus gordonii hppA (oligopeptide permease) mutant result from transcriptional effects on cshA adhesin gene expression
Microbiology 144, 127 (1998); https://doi.org/10.1099/00221287-144-1-127
/content/journal/micro/10.1099/00221287-144-1-127
/content/journal/micro/10.1099/00221287-144-1-127
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error