The phase-variable pilus-associated protein PilC is commonly expressed in clinical isolates of Neisseria gonorrhoeae, and shows sequence variability among strains

References

1. Bäckman M., Rudén A. -K. M., Bygdeman S. M., Jonsson A., Ringertz O., Sandström E. G. 1985; Gonococcal serovar distribution in Stockholm, with special reference to multiple infections and infected partners. Acta Pathol Microbiol Immunol Scand Sect B Microbiol 93:225–232
   [Google Scholar]
2. Bäckman M., Jacobson K., Ringertz S. 1995; The virgin population of Neisseria gonorrhoeae in Stockholm has decreased and antimicrobal resistance is increasing. Genitourin Med 71:234–238
   [Google Scholar]
3. Barritt D. S., Schwalbe R. S., Klapper D. G., Cannon J. G. 1987; Antigenic and structural differences among six proteins II expressed by a single strain of Neisseria gonorrhoeae. . Infect Immun 55:2026–2031
   [Google Scholar]
4. Buchanan T. M., Pearce W. A. 1976; Pili as mediators of the attachment of gonococci to human erythrocytes. Infect Immun 13:1483–1489
   [Google Scholar]
5. Diaz J. -L., Heckels J. E. 1982; Antigenic variation of outer membrane protein II in colonial variants of Neisseria gonorrhoeae P9. J Gen Microbiol 128:585–591
   [Google Scholar]
6. Hagblom P., Segal E., Billyard E., So M. 1985; Intragenic recombination leads to pilus antigenic variation in Neisseria gonorrhoeae. . Nature 315:156–158
   [Google Scholar]
7. Jonsson A. -B., Nyberg G., Normark S. 1991; Phase variation of gonococcal pili by frameshift mutation in pilC, a novel gene for pilus assembly. EMBO J 10:477–488
   [Google Scholar]
8. Jonsson A. -B., Pfeifer J., Normark S. 1992; Neisseria gonorrhoeae PilC expression provides a selective mechanism for structural diversity of pili. Proc Natl Acad Sci USA 89:3204–3208
   [Google Scholar]
9. Jonsson A. -B., Ilver D., Falk P., Pepose J., Normark S. 1994; Sequence changes in the pilus subunit lead to tropism variation of Neisseria gonorrhoeae to human tissue. Mol Microbiol 13:403–416
   [Google Scholar]
10. Jonsson A. -B., Rahman M., Normark S. 1995; Pilus biogenesis gene, pilC, of Neisseria gonorrhoeae: pilC1 and pilC1 are each part of a larger duplication of the gonococcal genome and share upstream and downstream homologous sequences with opa and pil loci. Microbiology 141:2367–2377
    [Google Scholar]
11. Knapp J. S., Tam M. R., Nowinsky R. C., Holmes K. K., Sandström E. G. 1984; Serological classification of Neisseria gonorrhoeae with use of monoclonal antibodies to gonococcal outer membrane protein I. J Infect Dis 150:44–48
    [Google Scholar]
12. Kupsch E. M., Knepper B., Kuroki T., Heuer I., Meyer T. F. 1993; Variable opacity (Opa) outer membrane proteins account for the cell tropisms displayed by Neisseria gonorrhoeae for human leukocytes and epithelial cells. EMBO J 12:641–650
    [Google Scholar]
13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
14. Lambden P. R., Heckels J. E., McBride H., Watt P. J. 1981; The identification and isolation of novel pilus types produced by variants of N. gonorrhoeae P9 following selection in vivo. FEMS Microbiol Lett 10:339–341
    [Google Scholar]
15. Mandrell R. E., Schneider H., Apicella M., Zollinger W., Rice P. A., Griffiss J. M. 1986; Antigenic and physical diversity of Neisseria gonorrhoeae lipooligosaccharides. Infect Immun 54:63–69
    [Google Scholar]
16. Nassif X., Lowy J., Stenberg P., O’Gaora P., Ganji A., So M. 1993; Antigenic variation of pilin regulates adhesion of Neisseria meningitidis to human epithelial cells. Mol Microbiol 8:719–725
    [Google Scholar]
17. Nassif X., Beretti J. -L., Lowy J., Stenberg P., O’Gaora P., Pfeifer J., Normark S., So M. 1994; Roles of pilin and PilC in adhesion of Neisseria meningitidis to human epithelial and endothelial cells. Proc Natl Acad Sci USA 91:3769–3773
    [Google Scholar]
18. Rahman M., Källström H., Normark S., Jonsson A. -B. 1997; PilC of pathogenic Neisseria is associated with the bacterial cell surface. Mol Microbiol 25:11–25
    [Google Scholar]
19. Rudel T., van Putten J. P. M., Gibbs C. P., Haas R., Meyer T. F. 1992; Interaction of two variable proteins (PilE and PilC) required for pilus-mediated adherence of Neisseria gonorrhoeae to human epithelial cells. Mol Microbiol 6:3439–3450
    [Google Scholar]
20. Rudel T., Boxberger H. -J., Meyer T. F. 1995a; Pilus biogenesis and epithelial cell adherence of Neisseria gonorrhoeae pilC double knock-out mutants. Mol Microbiol 17:1057–1071
    [Google Scholar]
21. Rudel T., Scheuerpflug I., Meyer T. F. 1995b; Neisseria PilC protein identified as type-4 pilus tip-located adhesin. Nature 373:357–359
    [Google Scholar]
22. Sandström E., Lindell P., Härfast B., Blomberg F., Ryden A. -C., Bygdeman S. 1985; Evaluation of a new set of Neisseria gonorrhoeae serogroup W-specific monoclonal antibodies for serovar determination. In The Pathogenic Neisseriae pp 26–30 Edited by Schoolnik G. K. Washington, DC: American Society for Microbiology;
    [Google Scholar]
23. Swanson J. 1973; Studies on gonococcus infection. IV. Pili: their role in attachment of gonococci to tissue culture cells. J Exp Med 137:571–589
    [Google Scholar]
24. Swanson J., Bergström S., Robbins K., Barrera O., Corwin D., Koomey J. M. 1986; Gene conversing involving the pilin structural genes correlates with pilus + to pilus − changes in Neisseria gonorrhoeae. . Cell 47:267–276
    [Google Scholar]
25. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
    [Google Scholar]
26. Virji M., Everson J. S. 1981; Comparative virulence of opacity variants of Neisseria gonorrhoeae. . Infect Immun 31:965–970
    [Google Scholar]
27. Virji M., Everson J. S., Lambden P. R. 1982; Effect of anti-pilus antisera on virulence of variants of Neisseria gonorrhoeae for cultured epithelial cells. J Gen Microbiol 128:1095–1100
    [Google Scholar]
28. Virji M., Alexandrescu C., Fergusson D. J. P., Saunders J. P., Moxon E. R. 1992; Variations in the expression of pili: the effect on adherence of Neisseria meningitidis to human epithelial and endothelial cells. Mol Microbiol 6:1271–1279
    [Google Scholar]
29. Virji M., Saunders J. P., Simms G., Makepeace K., Maskell D., Fergusson D. J. 1993; Pilus-facilitated adherence of Neisseria meningitidis to human epithelial and endothelial cells: modulation of adherence phenotype occurs concurrently with changes in primary amino acid sequence and the glycosylation status of pilin. Mol Microbiol 10:1013–1028
    [Google Scholar]
30. Virji M., Makepeace K., Peak I., Payne G., Saunders J. P., Ferguson D. J. P., Moxon E. R. 1995; Functional implications of PilC expression in meningococci. Mol Microbiol 16:1087–1097
    [Google Scholar]