f In situ identification of nocardioform actinomycetes in activated sludge using fluorescent rRNA-targeted oligonucleotide probes
- Authors: Markus Schuppler†,1, Michael Wagner, Georg Schön, Ulf B. Göbel‡
- Author for correspondence: Markus Schuppler. Tel: +49 351 463 8591. Fax: +49 351 463 8573. e-mail: email@example.com
- Microbiology, January 1998 144: 249-259, doi: 10.1099/00221287-144-1-249
- Subject: Environmental Microbiology
- Published Online:
Summary: Hitherto, few environmental samples have been investigated by a ‘full cycle rRNA analysis’. Here the results of in situ hybridization experiments with specific rRNA-targeted oligonucleotide probes developed on the basis of new sequences derived from a previously described comparative 16S rRNA analysis of nocardioform actinomycetes in activated sludge are reported. Application of the specific probes enabled identification and discrimination of the distinct populations of nocardioform actinomycetes in activated sludge. One of the specific probes (DLP) detected rod-shaped bacteria which were found in 13 of the 16 investigated sludge samples from various wastewater treatment plants, suggesting their importance in the wastewater treatment process. Another probe (GLP2) hybridized with typically branched filaments of nocardioforms mainly found in samples from enhanced biological phosphorus removal plants, suggesting that these bacteria are involved in sludge foaming. The combination of in situ hybridization with fluorescently labelled rRNA-targeted oligonucleotide probes and confocal laser scanning microscopy improved the detection of nocardioform actinomycetes, which often showed only weak signals inside the activated-sludge flocs.
Present address: Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, DürerStraße 24, D-01307 Dresden, Germany.
Present address: Institut für Medizinische Mikrobiologie und Hygiene, Medizinische Fakultät (Charité), Humboldt-Universitat Berlin, DorotheenStraße 96, D-10098 Berlin, Germany.
- Keyword(s): fluorescence in situ hybridization, 16S rRNA, activated sludge, nocardioform actinomycetes, confocal laser scanning microscopy
© Society for General Microbiology 1998 | Published by the Microbiology Society
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