f The Nhal antiporter of Saccharomyces cerevisiae mediates sodium and potassium eft: lux
- Authors: Maria A. BaAueIos, Hana Sychrová, Claudine Bleykasten-Grosshans, Jean-Luc Souciet, Serge Potier1
- 1Author for correspondence: Serge Potier. Tel: +33 3 88244151. Fax: +33 3 88358484. e-mail: p0tieragem.u-strasbg.fr
- First Published Online: 01 October 1998, Microbiology 144: 2749-2758, doi: 10.1099/00221287-144-10-2749
- Subject: Bioenergetics And Transport
- Issue Published:
SUMMARY: The NHAl gene of Saccharomyces cerevisiae, transcribed into a 3-5 kb mRNA, encodes a protein mediating Na+ and K+ efflux through the plasma membrane that is required for alkali cation tolerance at acidic pH. Deletion of the gene in a wild-type strain resulted in higher sensitivity to both K+ and Na+ at acidic pH. Measurements of cation loss in strains carrying deleted or overexpressed alleles of NHAl demonstrated its role in K+ and Na+ efflux. In addition, high K+ and Na+ efflux observed upon alkalinization of the cytoplasm implies a role of Nhalp in the regulation of intracellular pH. Moreover, the overexpression of ENA1 and NHAl genes in an enal-46-nhalb strain showed that the Nhal alkalication antiporter is responsible for growth on high concentrations of KCI and NaCl at acidic pH, and Ena alkali-cation ATPases are necessary at higher pH values. Both systems have a complementary action to maintain the intracellular steadystate concentration of K+ and Na+.
© Society Society for General Microbiology, 1998 | Published by the Microbiology Society
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/content/journal/micro/10.1099/00221287-144-10-2749dcterms_title,dcterms_subject-pub_serialIdent:journal/micro AND -contentType:BlogPost104
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