%0 Journal Article %A BaAueIos, Maria A. %A Sychrová, Hana %A Bleykasten-Grosshans, Claudine %A Souciet, Jean-Luc %A Potier, Serge %T The Nhal antiporter of Saccharomyces cerevisiae mediates sodium and potassium eft: lux %D 1998 %J Microbiology, %V 144 %N 10 %P 2749-2758 %@ 1465-2080 %R https://doi.org/10.1099/00221287-144-10-2749 %K Na'/H+ antiporter %K salt tolerance %K yeast %K K+/H+ antiporter %I Microbiology Society, %X SUMMARY: The NHAl gene of Saccharomyces cerevisiae, transcribed into a 3-5 kb mRNA, encodes a protein mediating Na+ and K+ efflux through the plasma membrane that is required for alkali cation tolerance at acidic pH. Deletion of the gene in a wild-type strain resulted in higher sensitivity to both K+ and Na+ at acidic pH. Measurements of cation loss in strains carrying deleted or overexpressed alleles of NHAl demonstrated its role in K+ and Na+ efflux. In addition, high K+ and Na+ efflux observed upon alkalinization of the cytoplasm implies a role of Nhalp in the regulation of intracellular pH. Moreover, the overexpression of ENA1 and NHAl genes in an enal-46-nhalb strain showed that the Nhal alkalication antiporter is responsible for growth on high concentrations of KCI and NaCl at acidic pH, and Ena alkali-cation ATPases are necessary at higher pH values. Both systems have a complementary action to maintain the intracellular steadystate concentration of K+ and Na+. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-144-10-2749