1887

Abstract

SUMMARY: Two different N-terminal extensions have been identified within class II bacteriocin precursors. The first one is a two-glycine-type leader peptide associated with a dedicated ATP-binding cassette transporter. The second is a signal peptide which directs the bacteriocin precursor to the general secretion machinery. Mesentericin Y105 is a class II anti-Listeria bacteriocin produced by Leuconostoc mesentemides Y105 via a dedicated transport system (DTS). To investigate heterologous expression systems capable of producing mesentericin Y105 in various hosts, two different secretion vectors were constructed. One of them, containing the mesentericin Y105 structural gene fused to the segment encoding the divergicin A signal peptide, was introduced into Escherichia coli, Leuconostoc subsp. and Lactococcus subsp. In E. coli, mesentericin Y105 production was linked to a putative periplasmic toxicity. To take advantage of this secretion system, the mesentericin Y105 precursor was also produced in E. coli. It was demonstrated that this pre-bacteriocin exhibited some antagonistic activity against Listeria. To allow for comparison between the two different transport systems, mesentericin Y105 production using the vector containing the mesentericin Y105 structural genem and i t s DTS transporter operon was examined. The production of mesentericin Y105 was monitored by a new fast purification method followed by MS analysis. It was shown that, in Leuconostoc, the production of mesentericin Y105 is enhanced via the DTS compared to the general secretion pathway.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-10-2845
1998-10-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/10/mic-144-10-2845.html?itemId=/content/journal/micro/10.1099/00221287-144-10-2845&mimeType=html&fmt=ahah

References

  1. Allison G.E., Ahn C, Stiles M.E., Klaenhammer T.R. 1995a; Utilisation of the leucocin A export system in Leuconostoc gelidum for production of a Lactobacillus bacteriocin.. FEMS Microbiol Lett 131:87–93
    [Google Scholar]
  2. Allison G.E., Worobo R.W., Stiles M.E., Klaenhammer T.R. 1995b; Heterologous expression of the lactacin F peptides by Carnobacterium piscicola LV17.. Appl Environ Microbiol 61:1371–1377
    [Google Scholar]
  3. Axelsson L., Holck A. 1995; The genes involved in production of and immunity to sakacin A, a bacteriocin from Lactobacillus sake Lb706.. J Bacteriol 177:2125–2137
    [Google Scholar]
  4. van Belkum M.J., Stiles M. 1995; Molecular characterization of genes involved in the production of the bacteriocin leucocin A from Leuconostoc gelidum. . Appl Environ Microbiol 61:3573–3579
    [Google Scholar]
  5. van Belkum M.J., Worobo R.W., Stiles M. 1997; Doubleglycine-type leader peptides direct secretion of bacteriocins by ABC transporters: colicin V secretion in Lactococcus lactis. . Mol Microbiol 23:1293–1301
    [Google Scholar]
  6. Bukhtiyarova M., Rongguang Y., Ray B. 1994; Analysis of the pediocin Ach gene cluster from plasmid pSMB74 and its expression in a pediocin-negative Pediococcus acidilactici strain.. Appl Environ Microbiol 60:3405–3408
    [Google Scholar]
  7. Chopin A., Chopin M.-G, Miollo-Batt A., Langella P. 1984; Two plasmid-determined restriction and modification systems in Streptococcus lactis. . Plasmid 11:260–263
    [Google Scholar]
  8. Coventry M.J., Gordon J.B., Alexander M., Hickey M.W., Wan J. 1996; A food-grade process for isolation and partial purification of bacteriocins of lactic acid bacteria that uses diatomite calcium silicate.. Appl Environ Microbiol 62:1764– 1769
    [Google Scholar]
  9. Franke C.M., Leenhout K.J., Haandrikman A.J., Kok J., Venema G., Venema K. 1996; Topology of LcnD, a protein implicated in the transport of bacteriocins from Lactococcus lactis. . J Bacteriol 176:1766–1769
    [Google Scholar]
  10. Fremaux C, Héchard Y., Cenatiempo Y. 1995; Mesentericin Y105 gene clusters in Leuconostoc mesenteroides Y105.. Microbiology 141:1637–1645
    [Google Scholar]
  11. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmid DNA.. J Mol Biol 166:557–580
    [Google Scholar]
  12. Hastings J.W., Sailer M., Johnson K., Roy K.L, Vederas J.C., Stiles M.E. 1991; Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum. . J Bacteriol 173:7491–7500
    [Google Scholar]
  13. Håvarstein L.S., Diep D.B., Nes I.F. 1995; A family of bacteriocin ABC transporters carry out proteolytic processing of their substrates concomitant with export.. Mol Microbiol 16:229–240
    [Google Scholar]
  14. Héchard Y., Derijard B., Letellier F., Cenatiempo Y. 1992; Characterization and purification of mesentericin Y105, an antiListeria bacteriocin from Leuconostoc mesenteroides. . J Gen Microbiol 138:2725–2731
    [Google Scholar]
  15. Higgins C.F. 1992; ABC transporters: from micro-organisms to man.. Annu Rev Cell Biol 8:67–113
    [Google Scholar]
  16. Holo H., Nes I.F. 1989; High-frequency transformation, by electroporation, of Lactococcus lactis subsp.cremoris grown with glycine in osmotically stabilized media.. Appl Environ Microbiol 55:3119–3123
    [Google Scholar]
  17. Horn N., Marténez M.I., Marténez J.M., Hernández P.E., Gasson M.J., Rodríguez J.M., Dodd H.M. 1998; Production of pediocin PA-1 by Lactococcus lactis using the lactococcin A secretory apparatus.. Appl Environ Microbiol 64:818–823
    [Google Scholar]
  18. Klaenhammer T.R. 1993; Genetics of bacteriocins produced by lactic acid bacteria.. FEMS Microbiol Rev 12: 39–86
    [Google Scholar]
  19. Kunji E.R.S., Mierau I., Hagting A., Poolman B., Konings W.N. 1996; The proteolytic systems of lactic acid bacteria.. Antonie Leeuwenhoek 70:187–221
    [Google Scholar]
  20. Leer R.J., van der Vossen J.M.B.M., van Giezen M., van Noort J.M., Pouwels P.H. 1995; Genetic analysis of acidocin B, a novel bacteriocin produced by Lactobacillus acidophilus. . Microbiology 141:1629–1635
    [Google Scholar]
  21. McCormick J.K., Worobo R.W., Stiles M.E. 1996; Expression of the antimicrobial peptide carnobacteriocin B2 by a signal peptide-dependent general secretory pathway.. Appl Environ Microbiol 62:4095–4099
    [Google Scholar]
  22. Maftah A., Renault D., Vignoles C, Héchard Y., Bressollier P., Ratinaud M.H., Cenatiempo Y., Julien R. 1993; Membrane permeabilization of Listeria monocytogenes and mitochondria by the bacteriocin mesentericin Y105.. J Bacteriol 175:3232–3235
    [Google Scholar]
  23. Marugg J.D., Gonzales C.F., Kunka B.S., Ledeboer A.M., Pucci M.J., Toonen M.Y., Walker S.A., Zoetmulder L.C., Vandenbergh P.A. 1992; Cloning, expression, and nucleotide sequence of genes involved in production of pediocin PA-1, a bacteriocin from Pediococcus acidilactici PAC 1.0.. Appl Environ Microbiol 58:2360–2367
    [Google Scholar]
  24. Mathieu F., Sudirman Suwhandi I., Rekhif N., Millére J.B., Lefebvre G. 1993; Mesenterocin 52, a bacteriocin produced by Leuconostoc mesenteroides subsp.mesenteroides FR52.. J Appl Bacteriol 74:372–379
    [Google Scholar]
  25. Muriana P.M., Klaenhammer T.R. 1987; Conjugal transfer of plasmid-encoded determinants for bacteriocin production and immunity in Lactobacillus acidophilus 88.. Appl Environ Microbiol 53:553–560
    [Google Scholar]
  26. O’Sullivan D., Klaenhammer T.R. 1993; High and low-copy- number Lactococcus shuttle cloning vectors with features for clone screening.. Gene 137:227–231
    [Google Scholar]
  27. Quadri L.E., Kleerebezem M., Kulpers O.P., De Vos W.M., Roy K.L, Vederas J.C., Stiles M.E. 1997; Characterization of a locus from Carnobacterium piscicola LV17B involved in bacteriocin production and immunity: evidence for global inducer-mediated transcriptional regulation.. J Bacteriol 179:6163–6171
    [Google Scholar]
  28. Raya R.R., Fremaux C., De Antoni G., Klaenhammer T.R. 1992; Site-specific integration of the temperate bacteriophage ɸadh into the Lactobacillus gasseri chromosome and molecular characterization of the phage (attP) and bacterial {attB) attachment site.. J Bacteriol 174:5584–5592
    [Google Scholar]
  29. Revol-Junelles A.M., Mathis R., Kier F., Fleury Y., Delfour A., Lefebvre G. 1996; Leuconostoc mesenteroides subsp.mesenteroides FR52 synthesises two distinct bacteriocins.. Lett Appl Microbiol 23:120–124
    [Google Scholar]
  30. Sambrook J., Fritsch E., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Schägger H., von Jagow G. 1987; Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.. Anal Biochem 166:368–379
    [Google Scholar]
  32. Stoddard G.W., Petzel J.P., van Belkum M.J., Kok J., McKay L.L. 1992; Molecular analyses of the lactococcin A gene cluster from Lactococcus lactis subsp.lactis biovar diacetylactis WM4.. Appl Environ Microbiol 58:1952–1961
    [Google Scholar]
  33. van der Vossen J.M.B.M., Kok J., Venema G. 1985; Construction of cloning, promoter-screening, and terminatorscreening shuttle vector for Bacillus subtilis and Streptococcus lactis. . Appl Environ Microbiol 50:540–542
    [Google Scholar]
  34. van der Vossen J.M.B.M., van der Lelie D., Venema G. 1987; Isolation and characterization of Streptococcus cremoris Wg2-specific promoters.. Appl Environ Microbiol 53:2452–2457
    [Google Scholar]
  35. Worobo R.W., van Belkum M.J., Sailer M., Roy K.L., Vederas J.C., Stiles M.E. 1995; A signal peptide secretion-dependent bacteriocin from Carnobacterium divergens. . J Bacteriol 177:3143–3149
    [Google Scholar]
  36. Zhang L.H., Fath M.J., Tai P.C., Kolter R. 1995; Genetic analysis of the colicin V secretion pathway.. Genetics 141:25–32
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-10-2845
Loading
/content/journal/micro/10.1099/00221287-144-10-2845
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error