f The transcriptional regulator gene phrR in Sinorhizobium meliloti WSM419 is regulated by low pH and other stresses
- Authors: Wayne G. Reeve, Ravi P. Tiwari, Cheryl M. Wong, Michael J. Dilworth, Andrew R. Glenn†
- Author for correspondence: Michael J. Dilworth. Tel: +61 8 9360 2112. Fax: +61 8 9360 6486. e-mail: firstname.lastname@example.org
- Microbiology, December 1998 144: 3335-3342, doi: 10.1099/00221287-144-12-3335
- Subject: Physiology And Growth
- Published Online:
Summary: The phrR gene in Sinorhizobium meliloti (previously known as Rhizobium meliloti) WSM419, directly downstream from actA, is induced by low pH or certain stresses (e.g. high concentrations of Zn2+, Cu2+, H2O2 or ethanol), but not in stationary phase or by other stresses (e.g. phosphate limitation, elevated temperature, high concentrations of sucrose or iron). A DNA fragment containing the wild-type phrR gene could not be cloned and inverse PCR was therefore used to amplify a 3·5 kb BamHI fragment containing phrR from the mutant S. meliloti TG2-6 (actA::Tn5). DNA fragments from a BamHI/SalI digest of the amplified product were cloned into pUK21 and sequenced. The phrR open reading frame contiguous to actA appears to code for a 15·2 kDa protein showing significant identity with the proteins encoded by y4wC and y4aM in Rhizobium sp. NGR234. All three proteins resemble transcriptional regulators in containing a DNA-binding helix-turn-helix motif similar to that reported for URF4 in Rhodospirillum rubrum and repressors in coliphage.
Present address: Vice Chancellery, University of Tasmania, Hobart, Tasmania, Australia.
© Society for General Microbiology 1998 | Published by the Microbiology Society
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