1887

Abstract

Summary: Genetic determinants of the temperate mycobacteriophage Ms6 required for chromosomal integration were identified. DNA sequence analysis of an -containing fragment revealed an ORF encoding a protein of 372 amino acid residues with a C-terminus similar to other conserved C-terminal regions typical of the phage integrase family. Comparison of the sequences of , and bacteria-prophage junctions and showed a 26 bp common core sequence, where recombination takes place, near the 5′ end of the integrase gene. Nucleotide sequence analysis of the chromosomal region showed that the core site overlaps the 3′ end of the tRNAgene. An integration-proficient plasmid vector was constructed and efficiently inserted at the tRNAgene of BCG and H37Ra. It was demonstrated that Ms6 and D29 integrative systems can be used in conjunction for inserting genes at multiple loci. The site-specific integration system of mycobacteriophage Ms6 is a new tool for mycobacterial genetic analysis and is poorly related to those of the L5 bacteriophage family.

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1998-12-01
2024-03-29
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References

  1. Abremski K.E., Hoess R.H. 1992; Evidence for a second conserved arginine residue in the integrase family of recom-bination proteins.. Protein Eng 5:87–91
    [Google Scholar]
  2. Anes E., Portugal I., Moniz-Pereira J. 1992; Insertion into the Mycobacterium smegmatis genome of the aph gene through lysogenization with the temperate mycobacteriophage Ms6.. FEMS Microbiol Lett 95:21–26
    [Google Scholar]
  3. Argos P., Landy A., Abremski K. & 9 other authors 1986; The integrase family of site-specific recombinases: regional similarities and global diversity.. EMBO J 5:433–440
    [Google Scholar]
  4. Boccard F., Smokvina T., Pernodet J.-L., Friedmann A., Guérineau M. 1989; The integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens is related to temperate bacteriophages.. EMBO J 8:973–980
    [Google Scholar]
  5. Brasch M.A., Pettis G.S., Lee S.C., Cohen S.N. 1993; Localization and nucleotide sequences of genes mediating site- specific recombination of the SLP1 element in Streptomyces lividans.. J Bacteriol 175:3067–3074
    [Google Scholar]
  6. Brown D.P., Idler K.B., Katz L. 1990; Characterization of the genetic elements required for site-specific integration of plasmid pSE211 in Saccbaropolyspora erythraea.. J Bacteriol 172:1877–1888
    [Google Scholar]
  7. Brown D.P., Idler K.B., Backer D.M., Donadio S., Katz L. 1994; Characterization of the genes and attachment sites for site-specific integration of plasmid pSElOl in Saccbaropolyspora erythraea and Streptomyces lividans.. Mol Gen Genet 242:185–193
    [Google Scholar]
  8. Campbell A.M. 1962; Episomes.. Adv Genet 11:101–145
    [Google Scholar]
  9. Campbell A.M. 1992; Chromosomal insertion sites for phages and plasmids.. J Bacteriol 174:7495–7499
    [Google Scholar]
  10. Canard B., Cole S.T. 1990; Lysogenic phages of Clostridium perfringens: mapping of the chromosomal attachment sites.. FEMS Microbiol Lett 66:323–326
    [Google Scholar]
  11. Christiansen B., Johnsen M.G., Stendby E., Vogensen F.K., Hammer K. 1994; Characterization of the lactococcal temperate phage TP901-1 and its site-specific integration.. J Bacteriol 176:1069–1076
    [Google Scholar]
  12. Christiansen B., Bronsdsted L., Vogensen F.K., Hammer K. 1996; A resolvase-like protein is required for the site-specific integration of the temperate lactococcal bacteriophage TP901-1.. J Bacteriol 178:5164–5173
    [Google Scholar]
  13. Craig N.L., Nash H.A. 1984; E. coli integration host factor binds to specific sites in DNA.. Cell 39:707–716
    [Google Scholar]
  14. Deininger P.L. 1983; Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis.. Anal Biocbem 129:216–223
    [Google Scholar]
  15. Dussurget O., Rodriguez M., Smith I. 1996; An ideR mutant of Mycobacterium smegmatis has derepressed siderophore production and an altered oxidative-stress response.. Mol Microbiol 22:535–544
    [Google Scholar]
  16. Haeseleer F., Pollet J.-F., Bollen A., Jacobs P. 1992; Molecular cloning and sequencing of the attachment site and integrase gene of the temperate mycobacteriophage FRAT1.. Nucleic Acids Res 20:1420
    [Google Scholar]
  17. Hauser M.A., Socca J.J. 1992; Site-specific integration of the Haemophilus influenzae bacteriophage HP1: location of the boundaries of the phage attachment site.. J Bacteriol 174:6674–6677
    [Google Scholar]
  18. Hou Y.M., Schimmel P. 1989; Evidence that a major determinant for identity of a transfer RNA is conserved in evolution.. Biochemistry 28:6800–6804
    [Google Scholar]
  19. Lee C.Y., landolo J.J. 1986; Integration of staphylococcal phage L54a occurs by site-specific recombination: structural analysis of the attachment sites.. Proc Natl Acad Sci USA 83:5474–5478
    [Google Scholar]
  20. Lee C.Y., landolo J.J. 1988; Structural analysis of staphylococcal bacteriophage 𝜙11 attachment sites.. J Bacteriol 170:2409–2411
    [Google Scholar]
  21. Lee M.H., Pascopella L., Jacobs W.R. Jr Hatfull G.F. 1991; Site-specific integration of mycobacteriophage L5: integration- proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis and bacille Calmette-Guerin.. Proc Natl Acad Sci USA 88:3111–3115
    [Google Scholar]
  22. Leong J.M., Nunes-DUby S.E., Oser A.B., Lesser C.F., Youderian P., Susskind M.M., Landy A. 1986; Structural and regulatory divergence among site-specific recombination genes of lambdoid phages.. J Mol Biol 189:603–616
    [Google Scholar]
  23. Lillehaug D., Birkeland N.K. 1993; Characterization of genetic elements required for site-specific integration of the temperate lactococcal bacteriophage 𝜙LC3 and construction of integrationnegative 0LC3 mutants.. J Bacteriol 175:1745–1755
    [Google Scholar]
  24. Madon J., Moretti P. 1987; Site-specific integration and excision of pMEAlOO in Nocardia mediterranei.. Mol Gen Genet 209:257–264
    [Google Scholar]
  25. Matsuura M., Noguchi T., Yamaguchi D., Aida T., Asayama M., Takahashi H., Shirai M. 1996; The sre gene (ORF469) encodes a site-specific recombinase responsible for integration of the R4 phage genome.. J Bacteriol 178:3374–3376
    [Google Scholar]
  26. Mazodier P., Thompson C., Boccard F. 1990; The chromo-somal integration site of the Streptomyces element pSAM2 overlaps a putative tRNA gene conserved among actinomycetes.. Mol Gen Genet 222:431–434
    [Google Scholar]
  27. Omer C.A., Cohen S.N. 1986; Structural analysis of plasmid and chromosomal loci involved in site-specific excision and integration of the SLP1 element of Streptomyces coelicolor.. J Bacteriol 166:996–1006
    [Google Scholar]
  28. Pascopella L., Collins F.M., Martin J.M., Lee M.H., Hatfull G.F., Stover C.K., Bloom B.R., Jacobs W.R. Jr 1994; Use of in vivo complementation in Mycobacterium tuberculosis to identify a genomic fragment associated with virulence.. Infect Immun 62:1313–1319
    [Google Scholar]
  29. Peña C.E.A., Stoner J.E., Hatfull G.F. 1996; Positions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site.. J Bacteriol 178:5533–5536
    [Google Scholar]
  30. Peña C.E.A., Lee M.H., Pedulla M.L., Hatfull G.F. 1997; Characterization of the mycobacteriophage L5 attachment site, attP.. J Mol Biol 266:76–92
    [Google Scholar]
  31. Pierson L.S., Kahn M.L. 1987; Integration of satellite bacteriophage P4 in Escherichia coli: DNA sequences of phage and host regions involved in site-specific recombination.. J Mol Biol 196:487–496
    [Google Scholar]
  32. Portugal I., Anes E., Moniz-Pereira J. 1989; Temperate mycobacteriophage from Mycobacterium smegmatis.. Acta Leprol 7:S243
    [Google Scholar]
  33. Ranes M.G., Rauzier J., Lagranderie M., Gheorghiu M., Gicquel B. 1990; Functional analysis of pAL5000, a plasmid from Mycobacterium fortuitum: construction of a ‘mini’ Mycobacterium-Escherichia coli shuttle vector.. J Bacteriol 172:2793–2797
    [Google Scholar]
  34. Rausch H., Lehmann M. 1991; Structural analysis of the actinophage 𝜙C31 attachment site.. Nucleic Acids Res 19:5187–5189
    [Google Scholar]
  35. Reiter W.D., Palm P., Yeats S. 1989; Transfer RNA genes frequently serve as integration sites for prokaryotic genetic elements.. Nucleic Acids Res 17:1907–1914
    [Google Scholar]
  36. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  37. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  38. Smith-Mungo L., Chan I.T., Landy A. 1994; Structure of the P22 att site. Conservation and divergence in the lambda motif of recombinogenic complexes.. J Biol Chem 269:20798–20805
    [Google Scholar]
  39. Snapper S.B., Melton R.E., Mustafa S., Kieser T., Jacobs W.R. Jr 1990; Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis.. Mol Microbiol 4:1911–1919
    [Google Scholar]
  40. Sprinzl M., Hartmann T., Weber J., Blank J., Zeidler R. 1989; Compilation of tRNA sequences and sequences of tRNA genes.. Nucleic Acids Res 17:1–172
    [Google Scholar]
  41. Stover C.K., de la Cruz V.F., Fuerst T.R. & 14 other authors 1991; New use of BCG for recombinant vaccines.. Nature 351:456–460
    [Google Scholar]
  42. Sun J., Inouye M., Inouye S. 1991; Association of a retroelement with a P4-like cryptic prophage (retronphage 𝜙R73) integrated into the selenocystyl tRNA gene of Escherichia coli.. J Bacteriol 173:4171–4181
    [Google Scholar]
  43. Thompson J.F., Moitoso de Vargas L., Koch R., Kahmann R., Landy A. 1987; Cellular factors couple recombination with growth phase: characterization of a new component in the lambda site-specific recombination pathway.. Cell 50:901–908
    [Google Scholar]
  44. Weisberg R.A. 1983; Site-specific recombination in phage lambda.. In Lambda 2 pp. 211–250 Hendrix W., Roberts J.W., Stahl F.W., Weisberg R.A. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  45. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors.. Gene 33:103–119
    [Google Scholar]
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