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Volume 144, Issue 4

Tandem organization and highly disparate expression of the two laccase genes and in the cultivated mushroom Agaricus bisporus Free

Abstract

Two non-allelic laccase genes ( and ) in have been mapped to the same cosmid clone and are close together, in tandem. The intergenic region consists of 1562 bp between the stop codon of and the start codon of . Differences between the 5′ non-coding regions of the two genes suggest the potential for their differential regulation. By employing competitive RT-PCR and specific primer pairs that discriminate between and , it has been shown that the level of mRNA is approximately 300 times higher than that of mRNA in malt extract liquid cultures; in compost cultures mRNA is almost 7000 times more abundant than mRNA.

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  • Published Online:
Keyword(s): Agaricus bisporus , differential regulation , laccase genes and polyphenol oxidase
© Society for General Microbiology 1998
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1998-04-01
2024-05-10
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Tandem organization and highly disparate expression of the two laccase genes lcc1 and lcc2 in the cultivated mushroom Agaricus bisporus
Microbiology 144, 1063 (1998); https://doi.org/10.1099/00221287-144-4-1063
/content/journal/micro/10.1099/00221287-144-4-1063
/content/journal/micro/10.1099/00221287-144-4-1063
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