f Citrate synthase and 2-methylcitrate synthase: structural, functional and evolutionary relationships
- Authors: Ursula Gerike, David W. Hough, Nicholas J. Russell, Michael L. Dyall-Smith, Michael J. Danson*
- *Author for correspondence: Michael J. Danson. Tel: + 44 1225 826509. Fax:. +44 1225 826779 e-mail: M.J.Danson@bath.ac.uk
- Microbiology, April 1998 144: 929-935, doi: 10.1099/00221287-144-4-929
- Subject: Biochemistry
- Published Online:
Following the complete sequencing of the Escherichia coli genome, it has been shown that the proposed second citrate synthase of this organism, recently described by the authors, is in fact a 2-methylcitrate synthase that possesses citrate synthase activity as a minor component. Whereas the hexameric citrate synthase is constitutively produced, the 2-methylcitrate synthase is induced during growth on propionate, and the catabolism of propionate to succinate and pyruvate via 2-methylcitrate is proposed. The citrate synthases of the psychrotolerant eubacterium DS2-3R, and of the thermophilic archaea Thermoplasma acidophilum and Pyrococcus furiosus, are approximately 40% identical in sequence to the Escherichia coli 2-methylcitrate synthase andalso possess 2-methylcitrate synthase activity. The data are discussed with respect tothe structure, function and evolution of citrate synthase and 2-methylcitrate synthase.
© Society for General Microbiology 1998 | Published by the Microbiology Society
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