@article{mbs:/content/journal/micro/10.1099/00221287-144-4-929, author = "Gerike, Ursula and Hough, David W. and Russell, Nicholas J. and Dyall-Smith, Michael L. and Danson, Michael J.", title = "Citrate synthase and 2-methylcitrate synthase: structural, functional and evolutionary relationships", journal= "Microbiology", year = "1998", volume = "144", number = "4", pages = "929-935", doi = "https://doi.org/10.1099/00221287-144-4-929", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-144-4-929", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "metabolism", keywords = "propionate", keywords = "citrate synthase", keywords = "2-methylcitrate synthase", abstract = "Following the complete sequencing of the Escherichia coli genome, it has been shown that the proposed second citrate synthase of this organism, recently described by the authors, is in fact a 2-methylcitrate synthase that possesses citrate synthase activity as a minor component. Whereas the hexameric citrate synthase is constitutively produced, the 2-methylcitrate synthase is induced during growth on propionate, and the catabolism of propionate to succinate and pyruvate via 2-methylcitrate is proposed. The citrate synthases of the psychrotolerant eubacterium DS2-3R, and of the thermophilic archaea Thermoplasma acidophilum and Pyrococcus furiosus, are approximately 40% identical in sequence to the Escherichia coli 2-methylcitrate synthase andalso possess 2-methylcitrate synthase activity. The data are discussed with respect tothe structure, function and evolution of citrate synthase and 2-methylcitrate synthase.", }