%0 Journal Article %A Gerike, Ursula %A Hough, David W. %A Russell, Nicholas J. %A Dyall-Smith, Michael L. %A Danson, Michael J. %T Citrate synthase and 2-methylcitrate synthase: structural, functional and evolutionary relationships %D 1998 %J Microbiology, %V 144 %N 4 %P 929-935 %@ 1465-2080 %R https://doi.org/10.1099/00221287-144-4-929 %K metabolism %K propionate %K citrate synthase %K 2-methylcitrate synthase %I Microbiology Society, %X Following the complete sequencing of the Escherichia coli genome, it has been shown that the proposed second citrate synthase of this organism, recently described by the authors, is in fact a 2-methylcitrate synthase that possesses citrate synthase activity as a minor component. Whereas the hexameric citrate synthase is constitutively produced, the 2-methylcitrate synthase is induced during growth on propionate, and the catabolism of propionate to succinate and pyruvate via 2-methylcitrate is proposed. The citrate synthases of the psychrotolerant eubacterium DS2-3R, and of the thermophilic archaea Thermoplasma acidophilum and Pyrococcus furiosus, are approximately 40% identical in sequence to the Escherichia coli 2-methylcitrate synthase andalso possess 2-methylcitrate synthase activity. The data are discussed with respect tothe structure, function and evolution of citrate synthase and 2-methylcitrate synthase. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-144-4-929