Citrate synthase and 2-methylcitrate synthase: structural, functional and evolutionary relationships Gerike, Ursula and Hough, David W. and Russell, Nicholas J. and Dyall-Smith, Michael L. and Danson, Michael J.,, 144, 929-935 (1998), doi = https://doi.org/10.1099/00221287-144-4-929, publicationName = Microbiology Society, issn = 1350-0872, abstract= Following the complete sequencing of the Escherichia coli genome, it has been shown that the proposed second citrate synthase of this organism, recently described by the authors, is in fact a 2-methylcitrate synthase that possesses citrate synthase activity as a minor component. Whereas the hexameric citrate synthase is constitutively produced, the 2-methylcitrate synthase is induced during growth on propionate, and the catabolism of propionate to succinate and pyruvate via 2-methylcitrate is proposed. The citrate synthases of the psychrotolerant eubacterium DS2-3R, and of the thermophilic archaea Thermoplasma acidophilum and Pyrococcus furiosus, are approximately 40% identical in sequence to the Escherichia coli 2-methylcitrate synthase andalso possess 2-methylcitrate synthase activity. The data are discussed with respect tothe structure, function and evolution of citrate synthase and 2-methylcitrate synthase., language=, type=