RT Journal Article SR Electronic(1) A1 Zé-Zé, Líbia A1 Tenreiro, Rogério A1 Brito, Luisa A1 Santos, Mário A. A1 Paveia, HelenaYR 1998 T1 Physical map of the genome of Oenococcus oeni PSU-1 and localization of genetic markers JF Microbiology, VO 144 IS 5 SP 1145 OP 1156 DO https://doi.org/10.1099/00221287-144-5-1145 PB Microbiology Society, SN 1465-2080, AB A physical map of the chromosome of Oenocccus oeni PSU-1 was constructed. This represents the first map for a strain of this species. A total of 37 restriction sites for the rare-cutting endonucleases Ascl, Fsel, Notl and Sfil were mapped on the chromosome, which was found to be circular with an estimated size of 1857 kb. Fragment order was determined using several approaches: analysis of partial and double digestions, two-dimensional pulsed-field gel electrophoresis, isolation of linking clones, and Southern hybridization with labelled restriction fragments both from PSU-1 and from O. oeni strain GM. Oenococcal genes alsS/alsD, mleA and mir, two phage attachment sites and recurrent sequences such as IS 1165-like elements and rrn loci were located on the physical map. Specific fragments hybridizing with gene probes from Lactococcus lactis, Leuconostoc mesenteroides and Bacillus subtilis were also identified. The two ribosomal operons have been precisely located and their transcription direction determined., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-144-5-1145