Cyanide hydrolysis in a cyanide-degrading bacterium, Pseudomonas stutzeri AK61, by cyanidase
Watanabe, Atsushi and Yano, Kazuyoshi and Ikebukuro, Kazunori and Karube, Isao,, 144, 1677-1682 (1998),
doi = https://doi.org/10.1099/00221287-144-6-1677,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= The cyanide-degrading bacterial strain AK61 was isolated from waste water at a metal-plating plant. The isolated strain was characterized by Gram-staining, quinone analysis, fatty acid profile and the API 20NE identification system, and identified as Pseudomonas stutzeri. Whole cells were able to degrade cyanide rapidly in a 1 mM solution containing no organic substances, and produced ammonia as a product. The induction of the cyanide-degrading activity of P. stutzeri AK61 did not depend on the presence of cyanide in the culture medium during growth. The cyanide-degrading enzyme was purified approximately 49-fold from a cell extract of P. stutzeri AK61. The enzyme had a Km of 1.7 mM for cyanide and a specific activity of 54.6 μmol ammonia produced min-1. The activity of the enzyme was optimal at 30 °C and pH 7.5. The results of SDS-PAGE, gel-filtration chromatography and NH2-terminal amino acid sequence analysis of the enzyme indicated that the functional enzyme was an aggregated protein consisting of a 38 kDa polypeptide. Like cyanidase (cyanide dihydratase), it was shown that the enzyme catalysed the hydrolysis of cyanide to ammonia and formate.,
language=,
type=