1887

Abstract

To understand the molecular mechanisms induced by stress that contribute to the development of tolerance in eukaryotic cells, the filamentous fungus has been chosen as a model system. Here, the response of germlings to heat shock is reported. The heat treatment dramatically increased the concentration of trehalose and induced the accumulation of mannitol and mRNA from the catalase gene . Both mannitol and catalase function to protect cells from different reactive oxygen species. Treatment with hydrogen peroxide increased germling viability after heat shock whilst mutants deficient in catalase were more sensitive to a 50 °C heat exposure. It is concluded that the defence against the lethal effects of heat exposure can be correlated with the activity of the defence system against oxidative stress.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-145-11-3229
1999-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/145/11/1453229a.html?itemId=/content/journal/micro/10.1099/00221287-145-11-3229&mimeType=html&fmt=ahah

References

  1. Brobst K. M., Lott C. E. Jr 1966; Determination of some compounds in corn syrup by gas-liquid chromatography of the trimethylsilyl derivatives. Cereal Chem 43:35–42
    [Google Scholar]
  2. Chaturvedi V., Flynn T., Niehaus W. G., Wong B. 1996a; Stress tolerance and pathogenic potential of a mannitol mutant of Cryptococcus neoformans. . Microbiology 142:937–943 [CrossRef]
    [Google Scholar]
  3. Chaturvedi V., Wong B., Newman S. L. 1996b; Oxidative killing of Cryptococcus neoformans by human neutrophils: evidence that fungal mannitol protects by scavenging reactive oxygen intermediates. J Immunol 156:3836–3840
    [Google Scholar]
  4. Chaturvedi V., Bartiss A., Wong B. 1997; Expression of bacterial mtlD in Saccharomyces cerevisiae results in mannitol synthesis and protects a glycerol-defective mutant from high-salt and oxidative stress. J Bacteriol 179:157–162
    [Google Scholar]
  5. Davidson J. F., Whyte B., Bissinger P. H., Schiestl R. H. 1996; Oxidative stress is involved in heat-induced cell death in Saccharomyces cerevisiae. . Proc Natl Acad Sci USA 93:5116–5121 [CrossRef]
    [Google Scholar]
  6. Donnely E., Barnett Y. A., McCullough W. 1994; Germinating conidiospores of Aspergillus amino acid auxotrophs are hypersensitive to heat shock, oxidative stress and DNA damage. FEBS Lett 355:201–204 [CrossRef]
    [Google Scholar]
  7. Godon C., Lagniel G., Lee J., Buhler J. M., Kieffer S., Perrot M., Boucherie H., Toledano M. B., Labarre J. 1998; The H2O2 stimulon in Saccharomyces cerevisiae. . J Biol Chem 273:22480–22489 [CrossRef]
    [Google Scholar]
  8. Jamieson D. J., Rivers S. L., Stephen D. W. S. 1994; Analysis of Saccharomyces cerevisiae proteins induced by peroxide and superoxide stress. Microbiology 140:3277–3283 [CrossRef]
    [Google Scholar]
  9. Jorge J. A., Polizeli M. L. T. M., Thevelein J. M., Terenzi H. F. 1997; Trehalases and trehalose hydrolysis in fungi. FEMS Microbiol Lett 154:165–171 [CrossRef]
    [Google Scholar]
  10. Kafer E. 1977; Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Adv Genet 19:33–131
    [Google Scholar]
  11. Kawasaki L., Wysong D., Diamond R., Aguirre J. 1997; Two divergent catalase genes are differentially regulated during Aspergillus nidulans development and oxidative stress. J Bacteriol 179:3284–3292
    [Google Scholar]
  12. Mager W. H., De Kruijff A. J. J. 1995; Stress-induced transcriptional activation. Microbiol Rev 59:506–531
    [Google Scholar]
  13. Moradas-Ferreira P., Costa V., Piper P., Mager W. 1996; The molecular defences against reactive oxygen species in yeast. Mol Microbiol 19:651–658 [CrossRef]
    [Google Scholar]
  14. Morgan R. W., Christman M. F., Jacobson F. S., Storz G., Ames B. N. 1986; Hydrogen peroxide-inducible proteins in Salmonella typhimurium overlap with heat shock and other stress proteins. Proc Natl Acad Sci USA 83:8059–8063 [CrossRef]
    [Google Scholar]
  15. Navarro R. E., Aguirre J. 1998; Posttranscriptional control mediates cell type-specific localization of catalase A during Aspergillus nidulans development. J Bacteriol 180:5733–5738
    [Google Scholar]
  16. Navarro R. E., Stringer M. A., Hansberg W., Timberlake W. E., Aguirre J. 1996; catA, a new Aspergillus nidulans gene encoding a developmentally regulated catalase. Curr Genet 29:352–359
    [Google Scholar]
  17. Piper P. 1997; The yeast heat shock response. In Yeast Stress Responses pp. 75–99Edited by Hohmann S., Mager W. H. Berlin & Heidelberg: Springer;
    [Google Scholar]
  18. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Santoro N., Thiele D. J. 1997; Oxidative stress responses in the yeast. In Yeast Stress Responses pp. 171–211Edited by Hohmann S., Mager W. H. Berlin & Heidelberg: Springer;
    [Google Scholar]
  20. Scandalios J. G. 1990; Response of plant antioxidant defense genes to environmental stress. Adv Genet 28:1–41
    [Google Scholar]
  21. Sweeley C. C., Bentley R., Makita M., Wells W. W. 1963; Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances. J Am Chem Soc 85:2497–2507 [CrossRef]
    [Google Scholar]
  22. Tauber A. I., Babior B. M. 1977; Evidence for hydroxyl radical production by human neutrophils. J Clin Invest 60:374–379 [CrossRef]
    [Google Scholar]
  23. Thevelein J. M. 1996; Regulation of trehalose metabolism and its relevance to cell growth and function. In The Mycota III, Biochemistry and Molecular Biology pp. 395–420Edited by Brambl R., Marzluf G. A. Berlin & Heidelberg: Springer;
    [Google Scholar]
  24. Wieser R., Adam G., Wagner A., Schuller C., Marchler G., Ruis H., Krawiec Z., Bilinski T. 1991; Heat shock factor-independent heat control of transcription of the CTT1 gene encoding the cytosolic catalase T of Saccharomyces cerevisiae. . J Biol Chem 266:12406–12411
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-145-11-3229
Loading
/content/journal/micro/10.1099/00221287-145-11-3229
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error