Mosaic structure of Shiga-toxin-2-encoding phages isolated from Escherichia coli O157:H7 indicates frequent gene exchange between lambdoid phage genomes
Johansen, Birgit K. and Wasteson, Yngvild and Granum, Per E. and Brynestad, Sigrid,, 147, 1929-1936 (2001),
doi = https://doi.org/10.1099/00221287-147-7-1929,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Shiga-toxin-2 (stx 2)-encoding bacteriophages were isolated from Norwegian Escherichia coli O157:H7 isolates of cattle and human origin. The phages were characterized by restriction enzyme analysis, hybridization with probes for toxin genes and selected phage DNA such as the P gene, integrase gene and IS1203, and by PCR studies and partial sequencing of selected DNA regions in the integrase to stx 2 region of the phages. The stx 2-phage-containing bacteria from which inducible phages were detected produced similar amounts of toxin, as shown by a Vero cell assay. The results indicate that the population of stx 2-carrying phages is heterogeneous, although the phages from epidemiologically linked E. coli O157:H7 isolates were similar. There appears to have been frequent recombination of stx 2 phages with other lambdoid phages.,
language=,
type=