1887

Abstract

This study examined the fine-scale genetic variation of the commercial mushroom, , over 2 years at two sites in France. One site was a meadow fertilized with horse manure and disturbed regularly by humans; the other was a Monterey cypress forest free of human disturbance. Altogether, 50 mushrooms were collected and analysed for mitochondrial and nuclear genetic variation marked by RFLPs and multilocus enzyme electrophoretic polymorphisms. Population samples from these two sites were genetically different and both sites contained high levels of genetic diversity. No identical genotypes were found at either site between the 2 years and there was little evidence for extensive vegetative clonality for this species. Contrary to expectations, very limited evidence of pseudohomothallic reproduction was found. Results from tests of Hardy–Weinberg equilibrium and genotypic equilibrium showed that outcrossing and recombination have played significant roles in both populations. The results demonstrated spatial-temporal genetic heterogeneity of in natural populations.

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2002-05-01
2024-04-19
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References

  1. Allen J. J., Moore D., Elliott T. J. 1992; Persistent meiotic arrest in basidia of Agaricus bisporus . Mycol Res 96:125–127 [CrossRef]
    [Google Scholar]
  2. Callac P. 1994; Prospections pour la recherche d’ Agaricus bisporus en France: contexte historique et scientifique, premiers résultats. Bull Soc Mycol Fr 110:145–165
    [Google Scholar]
  3. Callac P. 1995; Breeding of edible fungi with emphasis on the variability among French genetic resources of Agaricus bisporus . Can J Bot 73:S980–S986 [CrossRef]
    [Google Scholar]
  4. Callac P., Desmerger C., Kerrigan R. W., Imbernon M. 1997; Conservation of genetic linkage with map expansion in distantly related crosses of Agaricus bisporus . FEMS Microbiol 146:235–240 [CrossRef]
    [Google Scholar]
  5. Callac P., Hocquart S., Imbernon M., Desmerger C., Olivier J. M. 1998; Bsn-t allele from French field strains of Agaricus bisporus . Appl Environ Microbiol 64:2105–2110
    [Google Scholar]
  6. Callac P., Imbernon M., Guinberteau J., Pirobe L., Granit S., Olivier J.-M. 2000; Discovery of a wild Mediterranean population of Agaricus bisporus , and its usefulness for breeding work. Mushroom Sci XV:245–252
    [Google Scholar]
  7. Chang S. T. 1993; Mushroom biology: the impact on mushroom production and mushroom products. In Mushroom Biology and Mushroom Products pp 3–20 Edited by Chang S. T. Buswell J. A., Chiu S. W. Hong Kong: The Chinese University of Hong Kong Press;
    [Google Scholar]
  8. Guidot A., Debaud J. C., Marmeisse R. 2001; Between genet diversity and spatial distribution of above- and below-ground populations of the ectomycorrhizal fungus Hebeloma cylindrosporum . Mol Ecol 10:1121–1131 [CrossRef]
    [Google Scholar]
  9. Guinberteau J., Callac P., Boisselet P. 1998; Inventaire des communautés fongiques liées au Cupressus macrocarpa en zone littorale atlantique et données récentes sur les populations sauvages d’ Agaricus bisporus . Bull Soc Mycol Fr 114:19–38
    [Google Scholar]
  10. Hansen E. M., Hamelin R. C. 1997; Population structure of Basidiomycetes. In Structure and Dynamics of Fungal Populations pp 251–281 Edited by Worral J. Dordrecht: Kluwer;
    [Google Scholar]
  11. Hintz W. E. A., Anderson J. B., Horgen P. A. 1988; Physical mapping of the mitochondrial genome of the cultivated mushroom Agaricus brunnescens (= A. bisporus ). Curr Genet 14:43–49 [CrossRef]
    [Google Scholar]
  12. Imbernon M., Callac P., Granit S., Pirobe L. 1995; Allelic polymorphism at the mating type locus in Agaricus bisporus var. burnettii , and confirmation of the dominance of its tetrasporic trait. Mushroom Sci XIV:11–19
    [Google Scholar]
  13. Jin T., Horgen P. A. 1993; Further characterization of a large inverted repeat in Agaricus bisporus (= A. brunnescens ) and related species. Curr Genet 23:228–233
    [Google Scholar]
  14. Kerrigan R. W. 1995; Global genetic resources for Agaricus breeding and cultivation. Can J Bot 73:S973–S979 [CrossRef]
    [Google Scholar]
  15. Kerrigan R. W., Royer J. C., Baller L. M., Kohli Y., Horgen P. A., Anderson J. B. 1993; Meiotic behavior and linkage relationships in the secondarily homothallic fungus Agaricus bisporus . Genetics 133:225–236
    [Google Scholar]
  16. Kerrigan R. W., Billette C., Callac P., Velcko T. J. Jr 1996; A summary of allelic diversity and geographical distribution at six allozyme loci of Agaricus bisporus . In Mushroom Biology and Mushroom Products pp 25–35 Edited by Royse D. Proceedings of the Second International Conference; University Park, PA, USA:
    [Google Scholar]
  17. Kerrigan R. W., Carvalho D. B., Horgen P. A., Anderson J. B. 1998; The indigenous coastal Californian population of the mushroom Agaricus bisporus , a cultivated species, may be at risk of extinction. Mol Ecol 7:35–45 [CrossRef]
    [Google Scholar]
  18. Lange M. 1952; Species concept in the genus Coprinus . Dansk Bot Ark 14:1–140
    [Google Scholar]
  19. Mouyna I., Brygoo Y. 1993; Les champignons et la PCR. In La PCR: un Procede de Replication in vitro pp 335–341 Edited by Larzul D. Paris: Technique et Documentation; Lavoisier:
    [Google Scholar]
  20. Raper C. A., Raper J. R., Miller R. E. 1972; Genetic analysis of the life cycle of Agaricus bisporus . Mycologia 64:1088–1117 [CrossRef]
    [Google Scholar]
  21. Reynolds J., Weir B. S., Cockerham C. C. 1983; Estimating the coancestry coefficient: basis for a short-term genetic distance. Genetics 105:767–779
    [Google Scholar]
  22. Royse D. J., May B. 1982; Genetic relatedness and its application in selective breeding of Agaricus brunnescens . Mycologia 74:569–575 [CrossRef]
    [Google Scholar]
  23. Smith M. L., Bruhn J. N., Anderson J. B. 1992; The fungus Armillaria bulbosa is among the largest and oldest living organisms. Nature 356:428–431 [CrossRef]
    [Google Scholar]
  24. Summerbell R. C., Castle A. J., Horgen P. A., Anderson J. B. 1989; Inheritance of restriction fragment length polymorphisms in Agaricus brunnescens . Genetics 123:293–300
    [Google Scholar]
  25. Weir B. S., Cockerham C. C. 1984; Estimating F -statistics for the analysis of population structure. Evolution 38:1358–1370 [CrossRef]
    [Google Scholar]
  26. Xu J. 1995; Analysis of inbreeding depression in Agaricus bisporus . Genetics 141:137–145
    [Google Scholar]
  27. Xu J., Kerrigan R. W., Horgen P. A., Anderson J. B. 1993; Localization of the mating type gene in Agaricus bisporus . Appl Environ Microbiol 59:3044–3049
    [Google Scholar]
  28. Xu J., Horgen P. A., Anderson J. B. 1996a; Variation of mating interactions in Agaricus bisporus . Cultivated Mushroom Res Newsl 3:23–30
    [Google Scholar]
  29. Xu J., Horgen P. A., Anderson J. B. 1996b; Somatic recombination in the cultivated mushroom Agaricus bisporus . Mycol Res 100:188–192 [CrossRef]
    [Google Scholar]
  30. Xu J., Kerrigan R. W., Callac P., Horgen P. A., Anderson J. B. 1997; The genetic structure of natural populations of Agaricus bisporus , the commercial mushroom. J Hered 88:482–494 [CrossRef]
    [Google Scholar]
  31. Xu J., Kerrigan R. W., Sonnenberg A. S., Callac P., Horgen P. A., Anderson J. B. 1998; Mitochondrial DNA variation in natural populations of the mushroom Agaricus bisporus . Mol Ecol 7:19–33 [CrossRef]
    [Google Scholar]
  32. Zaykin D., Zhivotovsky L., Weir B. S. 1995; Exact tests for association between alleles at arbitrary numbers of loci. Genetica 96:169–178 [CrossRef]
    [Google Scholar]
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