1887

Abstract

Summary: In contrast to the failure of several previous workers to transduce streptomycin resistance, it was found that streptomycin indifference (one-step complete resistance) could quite reproducibly be transduced. For the expression of indifference after transduction, the infected bacteria must be allowed to divide at least once. This finding is assumed to reflect a segregation delay because of the recessive nature of the indifference locus. But from the fact that transduced indifference is phenotypically expressed from partial to complete resistance, the participation of a phenomic lag is also assumed.

In addition to the delay of phenotypic expression, several other aspects of transduction of indifference were studied and a close similarity of the behaviour of indifference to other chromosomal markers was found.

The transduction of one-step intermediate resistance into sensitive and into other one-step intermediate resistant recipients, however, was found to be difficult to demonstrate if it occurs at all. The difficulty of detecting resistant transductants is apparently not due to the killing of resistant bacteria in the presence of sensitive cells.

The difficulty of transducing intermediate resistance is assumed to be due to a selective disadvantage of the resistant, slow-growing transductants. The failure to find transductions even with slow-growing sensitive recipients may have been due to the fact that successful transductions yielded even slower growing resistant clones.

On the other hand, the transduction of second-step resistance into one-step resistant mutants was found to take place and the transduction of indifference into one-step resistant bacteria yielded slow-growing indifferent transductants.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-21-1-16
1959-08-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/21/1/mic-21-1-16.html?itemId=/content/journal/micro/10.1099/00221287-21-1-16&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1950; Methods of study of bacterial viruses. Meth. med. Res 2:1
    [Google Scholar]
  2. Alexander H. E., Leidy G. 1953; Induction of streptomycin resistance in sensitive Hemophilus influenzae by extracts containing desoxyribonucleic acid from resistant Hemophilus influenzae. J. exp. Med 97:17
    [Google Scholar]
  3. Baron L. S. 1953; Genetic transfers by means of Vi phage lysates. Cold Spr. Harb. Symp. quant. Biol 18:271
    [Google Scholar]
  4. Bryson V., Demerec M. 1955; Bacterial resistance. Amer. J. Med 18:1723
    [Google Scholar]
  5. Bryson V., Szybalski W. 1955; Microbial drug resistance. Advanc. Genet 7:1
    [Google Scholar]
  6. Cavalli L. L. 1952; Genetic analysis OF drug resistance. Bull. Wld Hlth Org 6:185
    [Google Scholar]
  7. Corey R. R., Starr M. P. 1957; Genetic transformation of streptomycin resistance in Xanthomonas phaseoli. J. Bact 74:146
    [Google Scholar]
  8. Demerec M. 1950; Reaction of populations of uninuclear organisms to extreme changes in environment. Amer. Nat 84:5
    [Google Scholar]
  9. Hotchkiss R. D. 1951; Transfer of penicillin resistance in pneumococci by the desoxyribonucleate derived from resistant cultures. Cold Spr. Harb. Symp. quant. Biol 16:457
    [Google Scholar]
  10. Hotchkiss R. D. 1952; The biological nature of the bacterial transforming factors. Exp. Cell Res. Suppl 2:383
    [Google Scholar]
  11. Hotchkiss R. D. 1957; Criteria for quantitative genetic transformation of bacteria. A Symposium on the Chemical Basis of Heredity p. 321 Baltimore: The Johns Hopkins Press;
    [Google Scholar]
  12. Hurwitz C., Rosanao C. L. 1958; Studies on mechanism of the streptomycin reaction. I. Phosphate reversal of the dihydrosteptomycin inactivation of Escherichia coli. J. Bact 75:11
    [Google Scholar]
  13. Iseki S., Sakai T. 1954; Transduction of biochemical properties in Salmonella E group. Proc. Jap. Acad 30:30
    [Google Scholar]
  14. Lederberg J. 1951; Streptomycin resistance: a genetically recessive mutation. J. Bact 75:11
    [Google Scholar]
  15. Lederberg J. 1956; Linear inheritance in transductional clones. Genetics 41:845
    [Google Scholar]
  16. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. J. Bact 63:399
    [Google Scholar]
  17. Lennox E. S. 1955; Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1:190
    [Google Scholar]
  18. Newcombe H. B. 1952; A comparison of spontaneous and induced mutations of Escherichia coli to streptomycin resistance and dependence. J. cell. comp. Physiol 39, Suppl. 1:13
    [Google Scholar]
  19. Newcombe H. B., Nyholm M. H. 1950; The inheritance OF streptomycin resistance and dependence in crosses of E. coli . Genetics 35:603
    [Google Scholar]
  20. Ozeki H. 1956; Abortive transduction in purine-requiring mutants of Salmonella typhimurium. Genetic studies with bacteria. Publ. Cameg. Inst 612:97
    [Google Scholar]
  21. Saz A. K., Eagle H. 1953; The co-killing of penicillin sensitive and penicillin resistant bacteria at low concentrations of the antibiotic. J. Bact 66:347
    [Google Scholar]
  22. Shimizu E. 1955; Transduction of biological properties in Salmonella typhimurium. Gumma J. Med. Sci 4:19
    [Google Scholar]
  23. Stocker B. A. D. 1956; Abortive transduction of motility in salmonella; a nonreplicated gene transmitted through many generations to a single descendent. J. gen. Microbiol 15:575
    [Google Scholar]
  24. Umbreit W. W., Smith P. H., Oginsky E. L. 1951; The action of streptomycin. V. The formation of citrate. J. Bact 61:595
    [Google Scholar]
  25. Watanabe T. 1954; Genetic studies on the mechanism of acquired streptomycin-resistance in microorganisms. Keio J. Med 3:193
    [Google Scholar]
  26. Watanabe T., Fukasawa T., Ushiba D. 1957; Probable absence of direct induction of bacterial resistance to streptomycin. J. Bact 73:770
    [Google Scholar]
  27. Zinder N. D. 1955; Bacterial transduction. J. cell. comp. Physiol 45, Suppl. 2:23
    [Google Scholar]
  28. Zinder N. D., Lederberg J. 1952; Genetic exchange in salmonella. J. Bact 64:679
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-21-1-16
Loading
/content/journal/micro/10.1099/00221287-21-1-16
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error