@article{mbs:/content/journal/micro/10.1099/00221287-22-2-323, author = "Hartman, P. E. and Loper, J. C. and Ĺ erman, D.", title = "Fine Structure Mapping by Complete Transduction Between Histidine-requiring Salmonella Mutants", journal= "Microbiology", year = "1960", volume = "22", number = "2", pages = "323-353", doi = "https://doi.org/10.1099/00221287-22-2-323", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-22-2-323", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: The growth characteristics, accumulations and genetic tests carried out by means of complete transduction for over 200 independently isolated histidine-requiring (his) mutants of Salmonella typhimurium are described. Sites of mutation engendering the histidine-requiring phenotype all lie within a short chromosomal region. This region is divisible into seven smaller regions, or gene loci, each correlated with a specific physiological phenotype. The gene loci appear to be linearly arranged and are linked in the order E, F, A, B, C, D and G. The five central loci are linked in an order corresponding to the sequence of reactions in the biosynthetic pathway for histidine. The functions of the two terminal loci, E and G, are unknown. The loci are nearly, or truly adjacent. A few of the sites of mutation are accurately mapped within a single gene. All the evidence is consistent with the view that processes involved in intra- and inter-genic recombination are identical. Some characteristics of multisite mutations are described. Several multisite mutations are interpreted as due to deletion of genetic material while one (his-57) is interpreted as due to an inversion, with or without concomitant deletion of an adjacent region. Single-site and multisite mutations exert specific effects on recombination frequencies in nearby regions.", }