RT Journal Article SR Electronic(1) A1 Kelly, M. A1 Clarke, Patricia H.YR 1962 T1 An Inducible Amidase Produced by a Strain of Pseudomonas aeruginosa JF Microbiology, VO 27 IS 2 SP 305 OP 316 DO https://doi.org/10.1099/00221287-27-2-305 PB Microbiology Society, SN 1465-2080, AB SUMMARY: A strain of Pseudomonas aeruginosa was obtained which was able to grow on acetamide or propionamide as sole source of carbon and nitrogen. When grown on these amides, whole bacteria and cell-free extracts rapidly hydrolysed acetamide, glycollamide, acrylamide and propionamide and slowly hydrolysed formamide and butyramide. N-Methylformamide, N-methylacetamide, N-ethylacetamide, N-acetylacetamide, N-methylpropionamide, N-ethylpropionamide, lactamide and methyl carbamate were found to be non-substrate inducers of the amidase when the organism was grown in succinate + ammonium chloride medium. N-Methylformamide, N-methylacetamide, lactamide and methyl carbamate did not inhibit propionamide hydrolysis by whole bacteria, but under the same conditions glycine amide, iodoacetamide and urea were effective inhibitors of amidase activity. N-Phenylacetamide, cyanoacetamide, glycine amide, sarcosine amide, β-hydroxy-propionamide and thioacetamide were neither substrates nor inducers of the amidase in this strain, but inhibited amidase induction by N-methylacetamide in succinate + ammonium chloride medium. Formamide also inhibited amidase induction under the same conditions., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-27-2-305