1887

Abstract

SUMMARY

This paper describes an investigation into the degree of homogeneity of the substance termed collocalia mucoid obtained from edible bird-nest. This material is a potent inhibitor of influenza virus haemagglutination and additional information is given here regarding its ability to neutralize infectivity. The crude material obtained by simple extraction was found to contain three components differing in molecular size and biological activity. Some separation of these was obtained by means of preparative ultracentrifugation. The slowest moving component differed from the others not only physically, but also in having much less inhibitor activity.

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/content/journal/micro/10.1099/00221287-31-1-31
1963-04-01
2024-04-19
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References

  1. Belyavin G., Westwood J. C. N., Please N. W., Smith W. 1951; Influenza haemagglutination: an evaluation of the photoelectric titration method. J. gen. Microbiol 5:546
    [Google Scholar]
  2. Burnet F. M., Stone J. D. 1947; The receptor destroying enzyme o. V. cholerae. Aust. J. exp. Biol. Med. Sci 25:227
    [Google Scholar]
  3. Cohen A., Belyavin G. 1959; Haemagglutination inhibition of Asian virus; a new pattern of response. Virology 7:59–74
    [Google Scholar]
  4. Gottschalk A., Fazekas de St Groth S. 1960; On the relationship between the indicator profile and prosthetic group of mucoproteins inhibitory for influenza virus haemagglutinin. J. gen. Microbiol 22:690
    [Google Scholar]
  5. Hirst G. K., Pickels E. G. 1942; A method for the titration of influenza haemagglu-tinins and influenza antibodies with the aid of the photoelectric densitometer. J. Immunol 45:273
    [Google Scholar]
  6. Howe C, Lee L. T., Rose H. M. 1960; Influenza virus sialidase. Nature, Lond 188:251
    [Google Scholar]
  7. Howe C, Lee L. T., Rose H. M. 1961; Collocalia mucoid: a substrate for myxovirus neuraminidase. Arch. Biochem. Biophys 95:512
    [Google Scholar]
  8. Lim K. A. 1954; A nomogram for 50 % end-point interpolations. J. Hyg., Camb 52:397
    [Google Scholar]
  9. Paul J. 1958; Determination of the major constituents of small amounts of tissue. Analyst 58:37
    [Google Scholar]
  10. Pusztai A., Morgan W. T. J. 1961; Studies in Immunochemistry, 18. The isolation and properties of a sialomucopolysaccharide possessing blood group Lea specificity and virus receptor activity. Biochem. J 78:135
    [Google Scholar]
  11. Shimojo H., Sugiura A., Akao Y., Enomoto C. 1959; Study of a non-specific haemagglutination inhibitor of influenza A/Asian/57 virus. Bull. Inst. publ. Hlth, Tokyo, 7:219
    [Google Scholar]
  12. Smith W., Westwood J. C. N., Belyavin G. 1951; A study of four virus strains isolated in 1951. Lancet ii:1189
    [Google Scholar]
  13. Sober H. A., Gutter F. J., Wycoff M. M., Peterson E. A. 1956; Chromatography of proteins. II. Fractionation of serum protein on anion exchange cellulose. J. Amer. ehem. Soc 78:756
    [Google Scholar]
  14. Tamm I., Horsfall F. L. 1952; A mucoprotein derived from human urine which reacts with influenza, mumps and Newcastle disease virus. J. exp. Med 95:71
    [Google Scholar]
  15. Thomson W. R. 1947; Use of moving averages and interpolation to estimate median effective dose. Bact. Rev 11:115
    [Google Scholar]
  16. Tiselius A., Hjertew S., Levin O. 1956; Protein chromatography on calcium phosphate columns. Arch. Biochem. Biophys 65:132
    [Google Scholar]
  17. Warren L. 1959; The thiobarbituric acid assay of sialic acids. J. biol. Chem 234:1971
    [Google Scholar]
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