1887

Abstract

SUMMARY

Methods of isolation and nutritional requirements of strains capable of growth on host-free media are described. Such strains retained their parasitic capacities for various Gram-negative bacteria after many transfers in host-free media. In parasite +host suspensions the spectrum of host specificity of various bdellovibrio strains was considerably wider than that obtained by examination for plaques on host lawns. The growth conditions therefore affect the capacity of the bdellovibrios to attach to and lyse host organisms. A proteolytic exoenzyme formed by certain bdellovibrio strains digested heat-killed, acid-treated or EDTA-treated host organisms, but did not affect intact host organisms. High phosphate concentrations inhibited exoenzyme activity. The morphological sequence of lysis induced by exoenzyme-forming parasitic bdellovibrio strains suggested that this lysis was a two-stage process: the first stage was specific attachment of bdellovibrios with damage to the cell wall of the host organisms ; the second stage was non-specific digestion of cellular components by exoenzyme produced by the bdellovibrios.

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/content/journal/micro/10.1099/00221287-40-3-317
1965-09-01
2024-04-24
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References

  1. Becker M. E., Hartsell S. E. 1954; Factors affecting bacteriolysis using Iysozyme in dual enzyme systems. Arch. Biochem. Biophys 53:402
    [Google Scholar]
  2. Becker M. E., Hartsell S. E. 1955; The synergistic action of Iysozyme and trypsin in bacteriolysis. Arch. Biochem. Biophys 55:257
    [Google Scholar]
  3. Bender H. 1963; Untersuchungen an Myxococcus xanthus. Partielle Lyse von Pullu-laria pullulans und einigen echten Hefen durch ein extracellulares Enzymsystem. Arch. Mikrobiol 45:405
    [Google Scholar]
  4. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by non-sulfur bacteria. J. Cell. comp. Physiol 49:25
    [Google Scholar]
  5. Fukasawa T., Jokura K., Kurahashi K. 1962; A new enzyme defect of galactose metabolism in Escherichia coli k-12 mutants. Biochem. biophys. Res. Comm 7:121
    [Google Scholar]
  6. Fukasawa T., Nikaido H. 1960; Formation of phage receptors induced by galactose in a galactose-sensitive mutant of Salmonella. Virology 11:508
    [Google Scholar]
  7. Horikoshi K., Shigeji I. 1959; Effect of lytic enzyme from Bacillus circulans and chitanase from Streptomyces sp. on Aspergillus oryzae . Nature, Lond 183:186
    [Google Scholar]
  8. Kohn A. 1960; Lysis of frozen and thawed cells of Escherichia coli by Iysozyme, and their conversion into spheroplasts. J. Bact 79:697
    [Google Scholar]
  9. McCrady M. H. 1918; Table for rapid interpretation of fermentation-tube results. Publ. Hlth J 9:169
    [Google Scholar]
  10. Mager J. 1964; A factor required for growth initiation of Pasteurella tularensis . Nature, Lond 203:898
    [Google Scholar]
  11. Noller E. C., Hartseix S. B. 1961a; Bacteriolysis of Enterobacteriaceae. Lysis of four lytic systems utilizing lysozyme. J. Bact 81:482
    [Google Scholar]
  12. Noller E. C., Hartsell S. E. 1961b; Bacteriolysis of Enterobacteriaceae. Pre- and co-lytic treatments potentiating the action of lysozyme. J. Bact 81:492
    [Google Scholar]
  13. Repaske R. 1958; Lysis of Gram-negative organisms and the role of versene. Biochim. biophys. Acta 30:225
    [Google Scholar]
  14. Salton M. R. J. 1958; The lysis of microorganisms by lysozyme and related enzymes. J. gen. Microbiol 18:481
    [Google Scholar]
  15. Stolp H., Petzold H. 1962; Untersuchungen uber einen obligat parasitischen Mikroorganismus mit lytischer Aktivität fur Pseudomonas Bakterien. Phytopathol. Z 45:364
    [Google Scholar]
  16. Stolp H., Starr M. 1963; Bdellovibrio bacteriovorus gen. et sp.n., a predatory, ectoparasitic and bacteriolytic microorganism. Antonie van Leeuwenhoek 29:217
    [Google Scholar]
  17. Sundarajan T. A., Rapin A. M. C, Kalckar H. M. 1962; Biochemical observations on E. colt mutants defective in uridine diphosphoglucose. Proc. natn. Acad. SciU.S.A 482187
    [Google Scholar]
  18. Yarmolinsky M. B., Wiesmeyer H., Kalckar H. M., Jordan E. 1959; Hereditary defects in galactose metabolism in Escherichia coli mutants, II. Galactose-induced sensitivity. Proc. natn. Acad. SciU.S.A 451786
    [Google Scholar]
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