1887

Abstract

SUMMARY:

Phages capable of changing the production of the ‘Tween’-splitting enzyme by lysogenic conversion of strains were isolated from lysogenic strains within the phage type 52/52A/80/81 complex.

Phages isolated from TW strains were able to block the production of the ‘Tween’-splitting enzyme when they lysogenized TW strains. When the TW receptor strains were originally lysogenic, double lysogenization occurred. This change in the ‘Tween’ reaction was shown to be an example of lysogenic conversion. Phages isolated from TW strains were found able, in some instances, to change TW strains to TW strains on lysogenization. This change in reaction was found to be due to loss of the converting phage carried by the recipient strains by prophage substitution. Where prophage substitution did not occur and the strains became doubly lysogenic, the strains remained TW.

In these experiments a change of the typing pattern was found after lysogenization. The phage type of the lysogenized strain became similar to that of the donor strain. Non-typable strains were found, doubly lysogenic for phages which between them blocked all the reactions to the typing phages employed. The significance of these findings is discussed, especially the use of lysogenized strains in experiments designed to investigate the role of ‘Tween’ negavity as a virulence factor.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-41-3-349
1965-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/41/3/mic-41-3-349.html?itemId=/content/journal/micro/10.1099/00221287-41-3-349&mimeType=html&fmt=ahah

References

  1. Asheshov E. H., Rippon J. E. 1959; Changes in typing pattern of phage-type 80 staphylococci. J. gen. Microbiol. 20:634
    [Google Scholar]
  2. Blair J. E., Williams R. E. O. 1961; Phage typing of Staphylococci. Bull. World Hlth Org. 24:771
    [Google Scholar]
  3. Comtois R. D. 1960; Changes in the phage-typing patterns of Staphylococci following lysogenization with a related group of Staphylococcus bacteriophages. Can. J. Microbiol. 6:491
    [Google Scholar]
  4. Freeman V. J. 1951; Studies on the virulence of bacteriophage-infected strains of Corynebacterium diphteriae. J. Bact. 61:675
    [Google Scholar]
  5. Jessen O., Rosendal K., Faber V., Hove K., Eriksen K. R. 1963; Some properties of Staphylococcus aureus, possibly related to pathogenicity. III. Bacteriological investigations of Staphylococcus aureus strains from 462 cases of bacteraemia. Acta path. microbiol. scand. 58:85
    [Google Scholar]
  6. Kjems E. 1955; Studies on streptococcal bacteriophages. I. Technique of isolating phage-producing strains. Acta path, microbiol. scand. 36:433
    [Google Scholar]
  7. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. J. Bact. 63:379
    [Google Scholar]
  8. Rosendal K., Bülow P., Jessen O. 1964; Lysogenic conversion in Staphylococcus aureus to a change in the production of extracellular ‘Tween’-splitting enzyme. Nature, Lond. 204:1222
    [Google Scholar]
  9. Rosendal K., Stenderup A., Helms P., Eriksen K. H. 1963; Staphylococcus aureus strains isolated in Danish hospitals from April 1st to December 31st 1960. Acta path, microbiol. scand. 58:72
    [Google Scholar]
  10. Rountree P. M. 1949; The serological differentiation of staphylococcal bacteriophages. J. gen. Microbiol. 3:164
    [Google Scholar]
  11. Rountree P. M. 1959; Changes in the phage-typing patterns of staphylococci following lysogenization. J. gen. Microbiol. 20:620
    [Google Scholar]
  12. Rountree P. M., Asheshov E. H. 1961; Further observations on changes in the phage-pattern of phage type 80/81 staphylococci. J. gen. Microbiol. 26:111
    [Google Scholar]
  13. Sierra G. 1957; Simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates. Antonie van Leeuwenhoek 23:15
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-41-3-349
Loading
/content/journal/micro/10.1099/00221287-41-3-349
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error