%0 Journal Article %A Bernheimer, A. W. %A Grushoff, Phyllis %T Cereolysin : Production, Purification and Partial Characterization %D 1967 %J Microbiology, %V 46 %N 1 %P 143-150 %@ 1465-2080 %R https://doi.org/10.1099/00221287-46-1-143 %I Microbiology Society, %X SUMMARY A method is described for production of the extracellular haemolytic growth-product of Bacillus cereus. The lytic substance, called ‘cereolysin’, can be purified by fractional precipitation with ammonium sulphate, density-gradient electrophoresis and gel filtration. The purified material is a labile protein of molecular weight about 52,000. It has a very high order of haemolytic activity, is lethal for mice, and resembles streptolysin O in being inhibited by cholesterol, but differs from streptolysin O in other respects. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-46-1-143