Cereolysin : Production, Purification and Partial Characterization Bernheimer, A. W. and Grushoff, Phyllis,, 46, 143-150 (1967), doi = https://doi.org/10.1099/00221287-46-1-143, publicationName = Microbiology Society, issn = 1350-0872, abstract= SUMMARY A method is described for production of the extracellular haemolytic growth-product of Bacillus cereus. The lytic substance, called ‘cereolysin’, can be purified by fractional precipitation with ammonium sulphate, density-gradient electrophoresis and gel filtration. The purified material is a labile protein of molecular weight about 52,000. It has a very high order of haemolytic activity, is lethal for mice, and resembles streptolysin O in being inhibited by cholesterol, but differs from streptolysin O in other respects., language=, type=