f The Effect of Environmental Conditions on the Motility of Escherichia coli
- Authors: J. ADLER, BONNIE TEMPLETON
- First Published Online: 01 February 1967, Microbiology 46: 175-184, doi: 10.1099/00221287-46-2-175
- Subject: Article
- Issue Published:
SUMMARY: A simple chemically defined medium for examining the motility of Escherichia coli K12 was designed. The essential components were: (1) a chelating agent to protect the motility against inhibition by traces of heavy metal ions; (2) a buffer to keep the pH value at the optimum between pH 6·0 and 7·5; (3) an energy source to stimulate the motility above that allowed by an endogenous energy source. Oxygen was required unless an energy source was provided which yielded energy anaerobically. A temperature optimum was determined.
A chemically defined growth medium capable of producing motile bacteria was devised. It was found that the presence of glucose or growth above 37° prevented synthesis of flagella.
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/content/journal/micro/10.1099/00221287-46-2-175dcterms_title,dcterms_subject-pub_serialIdent:journal/micro AND -contentType:BlogPost104
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