@article{mbs:/content/journal/micro/10.1099/00221287-46-2-175, author = "ADLER, J. and TEMPLETON, BONNIE", title = "The Effect of Environmental Conditions on the Motility of Escherichia coli", journal= "Microbiology", year = "1967", volume = "46", number = "2", pages = "175-184", doi = "https://doi.org/10.1099/00221287-46-2-175", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-46-2-175", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY A simple chemically defined medium for examining the motility of Escherichia coli k12 was designed. The essential components were: (1) a chelating agent to protect the motility against inhibition by traces of heavy metal ions; (2) a buffer to keep the pH value at the optimum between pH 6·0 and 7·5; (3) an energy source to stimulate the motility above that allowed by an endogenous energy source. Oxygen was required unless an energy source was provided which yielded energy anaerobically. A temperature optimum was determined. A chemically defined growth medium capable of producing motile bacteria was devised. It was found that the presence of glucose or growth above 37° prevented synthesis of flagella.", }